We discussed two gene therapy treatments: onefor Sickle Cell Disease (SCD) and one for Leber'sCongenital Amaurosis (LCA). Which one usesan ex-vivo approach?OLCASCDBoth of them Which of the following is not a component ofSanger sequencing?OddNTPs with fluorescent tagsPrimersRestriction enzymesO DNA template

Question 1 explantion: The component not typically used in Sanger sequencing is restriction enzymes.…

Answered: We discussed two gene therapy…

Biology Today and Tomorrow without Physiology (MindTap Course List)

5th Edition

ISBN:9781305117396

Author:Cecie Starr, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Christine Evers, Lisa Starr

Chapter10: Biotechnology

Section: Chapter Questions

Problem 1SQ

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Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
During pcr primers can: become graded by rnases be covslently linked to the template dtrand fall off the template once the polymerase leaves that sequence incorprste additional sequence not found in the template being copied
DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNases
DNA fragments can be separated by: O gel electrophoresis O autoradiography O x-ray crystallography O centrifugation
Which of the following statements is true of a Sanger sequencing reaction but not colony PCR? Select all that apply. Must contain only a single primer ddNTPs are added Uses a heat stable DNA polymerase Requires a pair of primers that bind to opposite strands Requires temperature cycling
Why is DNA polymerase a required part of PCR? O It synthesizes new DNA O It synthesizes proteins O It degrades the template DNA O It separates RNA from DNA MacBook Air
Why is the following statement fals? RNA polymerase is a dangerous enzyme to the cell because it does not proofread as well as DNA polymerase
which of the following sequencing techniques needs the highest coverage? o deep sequencing o de novo sequencing o resequencing o bisulfite sequencing
Which of the following cuts DNA like molecular scissors? Clustered regularly interpaced short palindromic repeats Cas (CRISPR associated ) proteins
What purpose do short DNA primers serve in PCR? O They determine which sequence of DNA is copied O They synthesize new DNA O They synthesize proteins O They separate RNA from DNA MacBook Air 80 888 F7 F6
Which of the following is NOT a characteristic of PCR primers? Short synthetic oligonucleotide Typically 18-25 bases in length Double stranded DNA Unique homology to the DNA template Sequence with ~50% G:C content

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