You want to study a biomolecule in the laboratory. You have ordered the synthetic genefrom a company and want to check that it has been introduced into your modelorganism. What is the proper order of experiments below for doing this?I. Sanger sequencingII. TransformationIII. Restriction digestIV. DNA purification from a single colonyI, II, III, IVI, IV, II, IIIIII, II, IV, III, III, IV, IIV, II, III, I

The process by which a specific gene sequence of interest is ligated and then the newly synthesized…

Answered: You want to study a biomolecule in the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Statement 1: Restriction enzymes are sequence-specific, which means that they only recognize a short yet unique sequence and only cleave the DNA in that paticular restriction site. Statement 2: The restriction enzyme used to cut the foreign DNA must be different from the restriction enzyme used to cut the restriction site of a plasmid. O Both statements are true. Both statements are false. Statement 1 is true. Statement 2 is false. Statement 1 is false. Statement 2 is true.
FIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved. 5'.GTAATCCGGATCCGAAATCCCCCGG... 3' 3.CATTAGGCCTAGGCTTTAGGGGGCCC... 5 FIGURE 2 Identify the most suitable enzymeto be used as a restriction enzyme forthis purpose. O Ligase O Smal O EcoRI О ЕсoRV
DNA scissors used in genetic engineering applications are called Endo nucleases Restriction enzymes Exo nucleases O DNases
For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.
(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.
The other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double stranded
Part 1. Gene Cloning 1) How is a gene inserted into a plasmid cloning vector? Be specific, but be brief. No more than 2 (non-run-on) sentences please, and do not exceed this space. 2) A geneticist has transformed a vector with an ampicillin resistance gene into E. coli cells and plated those cells on media containing ampicillin. She comes back 24 hours later and sees only blue colonies on the media. a) How does she know that the cells growing on the media were successfully transformed with the vector? b) What does it mean that the colonies are blue?
1. What is the purpose of restriction enzyme? 2. What is the purpose of the probe in DNA finger printing. 3. What is/are the sample that can be used for DNA finger printing? 4. Who is the culprit base on our interactive laboratory experiment?
Which of the following sequence shows the correct order of the steps involved in Recombinant DNA Technology I. Joining of a gene into a suitable vector. II. Introduction of this vector into a suitable organism. III. Multiplication or expression of a gene of interest. IV. Selection of transformed recombinant cells with a gene of interest. V. Identification and isolation of genes of interest. A. V, I, II, IV, III B. V, II, I, IV, III C. V, IV, III, II, I D. V, I, II, III, IV
Define and identify the words listed below: 1. restriction enzyme 2. topoisomerase 3. PCR
In 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.
During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification

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