You are attempting to create an E. coli mutant that lacks the enzyme to convert 1,3-Bisphosphoglycerate into 3-Phosphoglycerate. Normally you grow your E. coli in a medium withglucose as its only carbon source. What other compound would you need to add to your normalmedium in order to allow your mutant E. coli to grow?Pentose phosphate cyclePentose phosphateSedobestudose 2phosphateEnthrise-4phosphateGlyceraldehyde3phosphateFructoseErythroseGlycolysisGlucose phosphateFructose-6-phosphateFructose 1.6 diphosphateGlyceraldehyde-3-phosphate Dihydroxyacetone phosphate1,30iphosphoglycerate3PhosphoglycerateDynwate2PhosphoglyceratePhosphomolywateAcetyl coenzyme A-CrateIsocrate20Oxaloacetate,TCA cycleFumarateSuccinateYou don't need to add a second carbon source for your mutant to growPyruvateSuccinyl coenzyme A

E-Coli is a kind of bacterium of the Escherichia coli family. This bacterium is most commonly seen…

You are attempting to create an E. coli mutant that lacks the enzyme to convert 1.3- Bisphosphoglycerate into 3-Phosphoglycerate. Normally you grow your E. coli in a medium with glucose as its only carbon source. What other compound would you need to add to your normal medium in order to allow your mutant E. coli to grow? Pentose phosphate cycle Glycolysis Glucose phosphate Sedohetuse 2 phosphate Fructose-phosphate Fructose 1,6-dphosphate Glyceraldehyde-3-phosphate- 1.30phosphoglycerate Dihydroxyacetone phosphate

You are attempting to create an E. coli mutant that lacks the enzyme to convert 1.3- Bisphosphoglycerate into 3-Phosphoglycerate. Normally you grow your E. coli in a medium with glucose as its only carbon source. What other compound would you need to add to your normal medium in order to allow your mutant E. coli to grow? Pentose phosphate cycle Glycolysis Glucose phosphate Sedohetuse 2 phosphate Fructose-phosphate Fructose 1,6-dphosphate Glyceraldehyde-3-phosphate- 1.30phosphoglycerate Dihydroxyacetone phosphate

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 14P: Energetic of Fructose-1 ,6-bis P Hydrolysis (Integrates with Chapter 3.) The standard free energy...

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A solution of [U 14C] glucose-1-phosphate (specific activity = 16,000 cpm/mmole) was incubated with glycogen and glycogen phosphorylase, an enzyme which adds glucose units on to glycogen. Radioactivity was incorporated into the glycogen primer at a rate = 2550 cpm/min. The rate of the enzymatic reaction in units of mmole glucose incorporated per minute is: (a) 0.016 mmol/min (b) 0.57 mmol/min (c) 0.16 mmol/min (d) 5.7 mmol/min
Energetic of Fructose-1 ,6-bis P Hydrolysis (Integrates with Chapter 3.) The standard free energy change (G) for hydrolysis of fructose-1. 6-bisphosphate (FBP) to fructose-S-phosphate (F-6-P) and P: is -16.7 KJ/mol: FBP + H2O fructose-6-P + Pi The standard free energy change (G) for ATP hydrolysis is -30.5 KJ/mol: ATP + H2O ADP + Pj What is the standard free energy change for the phosphofructokinase reaction: ATP + fructose-6-P ADP + FBP b. What is the equilibrium constant for this reaction? c. Assuming the intracellular concentrations of [ATP] and (ADP] are maintained constant at 4 mM and 1.6 mM, respectively, in a rat liver cell, what will be the ratio of [FBP]/[fructose-6-P] when the phosphofructokinase reaction reaches equilibrium?
Match each of the enzymes involved in de novo pyrimidine synthesis with the correct description: _______ Dihydroorotate dehydrogenase _______ UMP synthase_______ Carbamoyl phosphate synthetase II _______ TMP synthase _______ CTP synthetase_______ Aspartate transcarbamoylase _______ PRPP synthetase (A) attaches an aspartate to an activated carbamoyl molecule(B) bifunctional enzyme that combines a nitrogenase pyrimidine ring to an activated ribose(C) catalyzes the formation of the activated ribose sugar(D) uses glutamine to add an amine to a preformed nucleotide(E) produces the first cyclic nitrogenase ring during de novo pyrimidine synthesis(F) combines bicarbonate and nitrogen from glutamine in the first step of pyrimidine ring synthesis (G) uses a folate derivative to add a methyl group to a preformed nucleotide
A liver cell undergoing gluconeogenesis is incubated in the presence of 14C labelled carbonic acid (H14CO3H). Soon after the incubation begins, the gluconeogenic products and intermediates from these cells are isolated. Where would you expect the radiolabelled carbon to be found? O C1 of lactate O C1 of glucose O Carbon dioxide O C3 of phosphoenol pyruvate O C1 of phosphoenolpyruvate
Which of these metabolic by-products can increase RER above 1.0? Ca2+ lactate H+ Pi
Choose the enzyme and cofactors involved in the reaction of fructose-1,6-bisphosphate to fructose-6-phosphate in gluconeogenesis. H H-TOPO Fo HOTH HDH HOH MOTOPOP K E H-TOH H OPO Problem 3 of 17 A B Q D E fructose-1,6-bisphosphatase phosphoglucose isomerase glucose-6-phosphatase phosphoglycerate kinase hexokinase
With respect to the following pairs of biomolecules which is produced via a mutarotation? D-Fructose and L-Fructose a-D-Fructose and 3-L-Glucose a-D-Mannose and 3-D-Mannose O a-D-Fructose and 3-L-Fructose O D-Fructose and D-Glucose
The reaction catalyzed by phosphorylase is readily reversible in vitro. At pH 6.8, the equilibrium ratio of orthophosphate to glucose 1-phosphate is 3.6. The value of ΔG°’ for this reaction is small because a glycosidic bond is replaced by a phosphoryl ester bond that has a nearly equal transfer potential. However, phosphorolysis proceeds far in the direction of glycogen breakdown in vivo. Suggest one means by which the reaction can be made irreversible in vivo.
Of course, one cannot stick a piece of tape to each carbon atom in the actual glucosemolecule. But one can do the next best thing: use radioisotopes to trace themovement of various atoms. Specifically, because carbon dioxide is a gas, usingradioactive C-14 allows for the easy detection of the 14CO2 that is given off.Suppose you introduce one glucose molecule that has had all of its carbons radioactivelylabeled. Suppose further that each step in glycolysis and in the citric acid cycle takes oneminute to complete (so glycolysis, for instance, takes ten minutes to complete, theconversion to acetyl CoA takes one minute, and the citric acid cycle takes eight minutes).Draw a plot of time (x-axis) versus radioactivity detected by a gas sensor (y-axis). Labelthe x-axis with enough time increments so it is clear at what minute the radioactivitywas detected. Use differing peak heights to give an indication of the amount ofradioactivity detected at that point.
What products are formed when the stachyose (refer to the photo) is hydrolyzed? sorbose, 2 molecules of tagatose and 2 molecules of fucose glucose, fructose, and 2 molecules of galactose glucose, fructose, and galactose glucose, 2 molecules of fructose and 2 molecules of galactose
Lactose is made up of D-galactose and one unit of D-glucose joined by a alpa-1,4-glycosidic bond and can be hydrolyzed by the enzyme lactase True False
3-phosphoglycerate serves as the precursor of the glycerol backbone during TAG biosynthesis. O True False