The synthesis of arginine by Nuerospora was determined by examining a number of mutant strains that were unable to synthesize the compound. Use the table of bacterial growth below to 1) determine the correct sequence of the synthesis pathway and 2) where in the synthesis pathway each mutation interrupts the synthesis. A “+" indicates growth. Nothing added to Succinate Ornithine added Strain Cirtulline Arginine Added added added growth medium Wild Mutant 1 Mutant 2 Mutant 3 Mutant 4
Q: What is the process by which bacteria convert nitrogen gas in the air to ammonia ?
A: Almost 80% of the atmosphere is made of nitrogen. It is an important constituent to make up bio…
Q: What is reverse DNA gyrase and why is it importantto hyperthermophiles?
A: Hyperthermophiles are high temperature loving bacteria. Most common of them is Thermotoga maritima.…
Q: What is the role of the origin of transfer during F+- and Hfr-mediated conjugation? What is the…
A: Conjugation is a process of transfer of genetic material between two bacteria. This transfer can…
Q: What is micropropagation? Describe the different in-vitro stages in the micropropagation of any…
A: Plant tissue culture is a technique in plant biotechnology. This technique involves the culturing of…
Q: What is the purpose of transgenesis
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: What is conjugation? What role do plasmids play in conjugation?
A: Bacteria are the most important microorganisms to the food processor. Some bacteria are beneficial…
Q: If you wanted to genetically engineer higher metabolic production of a target end product 'X', how…
A: There has been a tremendous advances made in the understanding of metabolic pathways. The…
Q: In order to transform a bacteria, the cell wall should be perforated either through physical or…
A: The cell wall is the very outer membrane of the cell. It has a rigid, semi-permeable protective…
Q: "the supplement that restored growth would be the molecule that the mutant strain could not…
A: Introduction: Mutant strain grew on insignificant medium with amino acids, mutants should not have…
Q: Where is the key difference between the reactions that result in the final product of fermentation…
A: Biomolecules are the biological molecules that are present inside the living organisms. These…
Q: You measure the zone of inhibition around the tetracycline disk as 14 mm. How would you report the…
A: Answer. Disk diffusion method It is a method used for the detection of susceptibility of various…
Q: The protein component of the transmembrane complex used by the salt-loving archaeobacterium…
A: Halobacterium halobium is a member of genus halobacterium of domain Archaea in the family of…
Q: In the experiment performed by Messelson and Stahl, bacterial cells grown in 1N-containing growth…
A: Watson and Crick proposed the semi-conservative model for the replication of DNA. Later, this was…
Q: Agrobacterium tumefaciens contains ------ plasmid inducing tumor in plants. Which of the following…
A: Agrobacterium tumefaciens is a bacteria which belongs to family Rizobiaceae.it is a rod shaped gram…
Q: Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase's…
A: Alfred Hershey and Martha chase did so in order to label the protein coat of virus with radioactive…
Q: what. is the hypothetical genetic control mechanism and iron acquisition system that C.diphtheriae.…
A: Diphtheria can infect the respiratory tract skin.
Q: What makes the species of Agrobacterium ideal for genetic engineering? Describe its characteristics…
A: In genetics, a vector is a biomolecule that serves as the carrier for the gene to be introduced in…
Q: The light-sensitive, protein component of the complex which pumps hydrogen ions out of the cell when…
A: Haloarchaea or halophilic archaea, are a class of the Euryarchaeota, found in water saturated or…
Q: Why might clinical medicine have an interest in understanding bacterial cell division at the…
A:
Q: Analyze the results shown in the table by finding: a. the order of the pathway products. b. the…
A: Asked: To find order of pathway product, order of genes and final product
Q: Ti plasmid carrying a foreign gene is inserted into which bacterium that infects a plant cell? a.…
A: The Ti plasmid vector is used in the world of plant genetic engineering with the aim of introducing…
Q: Explain the relationship between Piscirickettsiaceae and Alteromonadaceae. Which of the two bacteria…
A: In the deep sea microbial community, Alteromonadaceae is one of the indigenous bacterias that have a…
Q: In what ways does conjugation create bacterial cells that are at least partially diploid? Is this a…
A: Conjugation is the process of transfer of genetic material from one bacterium to another by direct…
Q: ou isolated a new microorganism that has single cells and appear to lack a nucleus.Chemical analysis…
A: Three domains of life are Archaea, Bacteria, and the Eucarya. Prokaryotes include domain bacteria…
Q: The peptidoglycan of bacteria has been compared with the chainmail worn beneath medieval knight’s…
A: Analogous functions are defined as the organs of different animals with different structures but…
Q: In a rapidly changing environment, which bacterial population would likely be more successful: one…
A: In a rapidly changing environment, the availability of nutrients, climatic conditions keep on…
Q: Bacterium Q had the lab results below. What can you tell me about the protein metabolism of this…
A: Test results Gelatin tube- Solid Urea agar slants- fushia Phenylalanine slants- no color Peptone…
Q: How might genes, such as those responsible for drug resistance, be transferred between bacterial…
A: According to the question, we have to explain the process by which the genes, responsible for drug…
Q: peptidoglycan can of bacteria has been campared with the chain mail worn beneath a medieval knights…
A: Peptidoglycan is the major component of cell walls that helps bacteria to survive in harsh…
Q: The non-protein component of the transmembrane complex used by the salt-loving archaeobacterium…
A: Halobacterium halobium is a prokaryotic organism that is found in the places where salt content is…
Q: Describe ,A Hfr bacterium mates with an F- plasmid containing bacterium. What
A: PLASMIDS- It was discovered that maleness in bacteria is determined by a transmissible genetic…
Q: You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A…
A: Bacteriophage : these are the viruses that attack on barterial cells and use them to produce more…
Q: What is the purpose of Agrobacterium cell duplication?
A: Agrobacterium tumefaciens is a pathogenic bacteria that usually causes crown gall disease in plants.…
Q: Determine the genotype for the bacteria strains shown below, and then classify them as nutritional…
A: Mutations that result in a change in the biochemical pathways and therefore biochemistry of a cell…
Q: After preparing cultures and completing a six-hour growth and viability trial, you notice that your…
A: Yeasts are little, single-celled plants. They are individuals from the fungus family which…
Q: Is the genus alcaligenes gelatin negative or postive?
A: Prokaryotes are single-celled microscopic organisms belongs to the domain bacteria. A prokaryotic…
Q: Why are regulatory mutants used to increase the production of glutamic acid by Corynebacterium…
A: Corynebacterium glutamicum is a rod-shaped gram-negative bacteria of the Corynebacteriaceae family…
Q: Why is E. coli O157:H7 an organism of concern in contaminated foods? The strain is represented as…
A: E.coli is a prokaryotic organism (bacteria) found in intestine of animals including humans, this…
Q: What are the reagents and materials used/needed in the extraction of invertase from yeast? for a…
A: Invertase is the enzyme that catalyzes the hydrolysis of the bond between sucrose and breakdown into…
Q: A conjugate was incubated with a mixture of lysosomal enzymes and no drug release was observed in…
A: Lysosomes contain a wide variety of hydrolytic enzymes including proteases, nucleases, glycosidases,…
Q: suggest a strategy that you might employ to isolate all of the genes involved in nitrogen fixation…
A: Nitrogen fixation is the process by which the free form of atmospheric nitrogen is converted or…
Q: In a nutrient medium that lacks histidine, a thin layer of agar containing ~109 Salmonella…
A: Ames Test:It is a biological assay that is used in assessing the mutagenic potential of the given…
Q: What is meant by "transformation" and how does Agrobacterium transform a plant cell? What are the…
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Discuss bacterial conjugation and the three mating types of bacteria (F-, F+, and Hfr).
A: Transfer of genes in prokaryotes occurs through horizontal gene transfer mechanisms such as…
Q: What is bacterial conjugation and how was it discovered?
A: Horizontal gene transfer (HGT) is defined as the exchange of genetic material between organisms.…
Q: Engineered bacteria are commonly used and cultured in pharmaceutical laboratories to produced drugs…
A: Insulin is a polypeptide hormone produced by the β-cells of islets of Langerhans of the pancreas. It…
Q: Which of the following questions would be most appropriate to investigate whether the proteolytic…
A: Proteolytic enzymes : Proteases occur in all organisms from prokaryotes to eukaryotes and to virus .…
Q: You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You…
A: INTRODUCTION In chemistry, pH traditionally denoting "ability of hydrogen" is a scale used to…
Q: Why is Agrobacteriummediated genetic engineering transformation inplants considered as natural…
A: Agrobacterium is a gram-negative bacteria genus that uses the HGT (horizontal gene transfer) method…
![The synthesis of arginine by Nuerospora was determined by examining a number of
mutant strains that were unable to synthesize the compound. Use the table of
bacterial growth below to 1) determine the correct sequence of the synthesis pathway
and 2) where in the synthesis pathway each mutation interrupts the synthesis. A “+"
indicates growth.
Nothing
added to
Succinate
Ornithine
added
Strain
Cirtulline
Arginine
Added
added
added
growth
medium
Wild
Mutant 1
Mutant 2
Mutant 3
Mutant 4](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe5d41aff-1864-4b78-ba7a-7ca0774ffdb6%2F8ac63c5b-860c-4bcb-9aee-fd79ffda48f7%2Fhgs10k8.png&w=3840&q=75)
![](/static/compass_v2/shared-icons/check-mark.png)
Trending now
This is a popular solution!
Step by step
Solved in 4 steps with 2 images
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
- One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/Under cellular conditions, dATP can be oxidized to its 2-hydroxy form, which is a substrate for an enzyme that converts 2-OH-dATP to 2-OH-dAMP + PPi. Explain why compounds that inhibit the activity of this enzyme would be eff ective anticancer agents.Which among statements A-D is not applicable to endospore formation? A) O It is likely to occur during the log phase of growth. B) O Formation of a mother cell occurs during the process. C) OIt is a property found in members of genus Bacillus and genus Clostridium. D) O Dipicolinic acid and calcium ions play an important role in making the spore highly resistant to environmental conditions, E) OA-D are all are applicable to endospore formation
- Which among A-D is false regarding growth and/or analysis of a bacterial culture? A) O Fed-batch growth: typically results in an increased yield of cells over that obtained with batch growth B) O Auxotroph: requires addition of specific growth factors in order to grow C) O Prototroph: typically the wild type strain that possesses all the features indicative of the species D) O None of A-D is false; all are true statementsGram-negative bacteria are surrounded by two membrane bilayers separated by a space termed the periplasm. The periplasm is a multipurpose compartment separate from the cytoplasm. The periplasm has a distinct oxidizing environment that allow certain key protein structural features to be formed. Can you identify an amino acid(s) that would be affected by this oxidizing environment? How would it be affected, and what structural features would be sensitive to this environment? Can you discuss the implications of this from a standpoint of recombinant protein expression?The pathway shown below is used by a fungus to create Arginine from a precursor starter molecule. A nonsense mutation occurs in the gene that encodes enzyme 2. This fungus is grown on media that only contains the precursor. Discuss the effects of this mutation on:(i) The function of Enzyme 2 (ii) The potential of the fungus to produce Ornithine
- b. Neurospora has an arginine amino acid synthesis pathway shown below: ornithine citrulline arginine NH H2N H2N OH H2N HO HO NH2 NH2 ÑH2 Enzyme A Enzyme B Enzyme C ↑ ↑ Gene A Gene B Gene C Suppose I have a neurospora strain that has a mutation such that it will not grow unless I supplement the media (food) with arginine (but not with citrulline or ornithine). What gene is mutated? Explain your reasoning. C. Suppose I take the strain above that only grows with arginine supplements and cross it to a different mutant Neurospora strain that grows with arginine and citrulline supplements but not ornithine supplements. Assming genes A, B and C are unlinked and there is only one mutation per strain: i) What percentage of the progeny will grow on ornithine? ii) What percentage on citrulline? iii) What percentage on arginine? Show your work for i), ii) and iii). |Chlamydia are pathogenic bacteria that must be grown within a eukaryotic host cell. They rely on the host cell for many nutrients, including nucleotides, lipids, and amino acids. It is thus no surprise that when grown in co-culture with a host depleted of any of these metabolites, chlamydial growth declines. However, high levels of certain amino acids also inhibit chlamydial growth. Specifically, the amino acids leucine, isoleucine, methionine, and phenylalanine slow down the growth by inhibiting the amino acid transporter called BrnQ. On the other hand, if valine is present in the medium these amino acids have no effect on BrnQ. Discuss how this might be possible12) Identify the most correct choice: a) All fungi reproduce by binary fission. b) Fungi are prokaryotes. 0 c) Fungi obtain nourishment via photosynthesis. d) Fungi are eukaryotes 13) A circular piece of DNA has one unique Xhol site (only one). Therefore digestion of that DNA with Xhol enzyme results in the production of two bands on an agarose gel. () True False
- 1) The inactivation of Staphylococcus aureus was studied in pomegranate juice heated at 75°- 95°C. The slopes for the inactivation of this bacteria were calculated from the linear lines in semi-log graphical paper. The slope values were presented in Table 1. a) Determine the temperature change needed to inactivate this bacteria by 90%. b) Temperature change to inactivate Bacillus cereus in pomegranate juice by 90% at the same temperature range was 29°F. At 75°-95°C temperature range, which bacteria will be more affected by the temperature changes? Commend on your answer in one sentence Table 1- Slope values for the inactivation of Staphylococcus aureus in pomegranate juice heated at various temperature Temp. (°C) 75 80 85 95 -Slope x 10² (min-¹) 2.222 3.704 5.000 13.158Within the light organ, bacteria are protected and nourished, and rapidly increase in number. At night, theyprovide the light needed for counterillumination. But by dawn, as the squid prepares to hide in the sand forthe day, it ejects about 95% of the bacteria from the light organ. a) Write a possible explanation for why eliminating 95% of the bacteria present in the light organ isnecessary. b) Once the light organ is colonized, the squid no longer takes in Vibrio fischeri or other bacteria from theenvironment. Explain how a sufficiently large population of bacteria can be maintained in the light organto produce light.The ribosome is the target for many important antibiotics. These drugs must discriminate between bacterial and eukaryotic ribosomes to achieve drug specificity and toxicity. For the two common antibiotics below, what is their mechanism of action and why are they more toxic to bacteria than eukaryotes? a) Tetracycline b) Erythromycin