Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.
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INSTRUCTION: Give the principle or significance of the following practices:
NOTE: *please add references*
1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them.
2. Flaming the inoculating needle or loop before and after inoculation.
3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.
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- MATCHING TYPE. Match the phlebotomy materials found in Column A to the Collection Method found in Column B. Column A: Materials 1. Cotton 2. Plain glass tube3. Red top glass tube4. Gauge 23 syringe5. Two-way needle6. Adapter7. Lancet8. Lavender top plastic tube9. Tourniquet10. Micropore Column B: Collection MethodA. Syringe MethodB. Evacuated SystemC. Winged CollectionD. Capillary CollectionE. All of the aboveF. Syringe and Evacuated onlyG. Evacuated and WingedProcedure: 1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva to each tube. Mix thoroughly. 2. Place the first tube in ice water, the 2nd tube leave at room temperature, the 3rd tube in 40°C , the 4th tube at 60°Cwater bath and the 5th tube boil for 2 minutes.. 3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4th tube allows to stand for 30 minutes after heating for 2 minutes. 4. Test the contents of each tube with iodine and benedict’s tests.困 Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following is/are TRUE the use of laboratory instruments and regarding the use laboratory instruments equipment in regards to making of culture media? " and equipment? * Air bubbles on the surface of agar plates Hot plates can be used to sterilize the inoculating loop and needle. can be removed by fanning them with the luminous flame of the bunsen burner. If the agar surface is wet, it can be dried by A drying oven is used to sterilize nutrient agar, a solid culture medium. heating at 30-40 deg C in the drying oven before inoculation is done. The refrigerator is used to grow psychrophiles. All media taken from the refrigerator should be warmed to room temperature before use, P Type here to search 7:11 pm 30/09/2021 ヘロG) ENG 近
- Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. VialsDiscuss the quality control issues that affect phlebotomy or that phlebotomy is subject to.Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
- Topic: Microbiological Examination of Non-sterile Products 2. The analyst unknowingly contaminated the product with Staphylococcus aureus organisms. Effect: Rationale:Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinetDirections: Prepare the following strength solutions: 360 mL of 1/4 strength Ensure tube feeding through a percutaneous endoscopic (PEG) tube over 4 hours. The nurse would add mL of water to mL of tube feeding to get 360 mL of 1/4 strength tube feeding.
- The client complains of nausea. You obtain an new order for prochlorperazine 25 mg rectal suppository every 12 hours PRN nausea, vomiting. Available: prochlorperazine 25 mg rectal suppository. How many suppositories will you administer to the client in your 8-hour shift?General Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference. Causative Agent and Disease Profile for S. aureus Template and Example ITEM MSM PROFILE MICROBIAL PROFILE I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY G MICROSCOPIC IMAGE II DISEASE PROFILE Scalded skin syndrome A DISEASE/S Skin and Wound Infections Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…Prepare the following strength solutions: 360 mL of 1/4 strength Ensure tube feeding through a percutaneous endoscopic (PEG) tube over 4 hours. The nurse would add____ mL of water to _____ mL of tube feeding to get 360 mL of 1/4 strength tube feeding.