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- 1. What are the different factors that affects the quality of water? 2. What is the role of a coagulating agent in the process of purification?10. A(n)------------------------------- is a solution that moderates changes in pH.2. Explain why there is minimal change in the pH when a strong acid is added to a buffer as opposed to when it is added in water.
- 4. What is the molecular nature of the substance responsible for the acid-fast property of an organism?4. What is the region/point where AA is predominantly present as a (-2)-charged species?5. The effective buffering range for the amino acid in the acidic region is?6. What is the region/point where the solution has a 50:50 percent mixture of the (0) and the (-1) species?10. Why would it be inaccurate to say that a buffer is a solution that maintains a constant pH? K/U
- II. Was the buffer better at providing H* or binding H* ?. Explain your answer.4. Tabulate the similarities and differences between an acidic and basic buffer solutions1. For each of the following separation methods covered in class, describe the basics of how that technique works, and what difference in material properties is used to separate molecules by that technique (for example: molecular size/MW, solubility differences, surface charge, ...) ultrafiltration, centrifugation, size-exclusion chromatography, isoelectric focusing. reverse osmosis, adsorption (physisorption), ion exchange, dialysis, affinity chromatography, electrophoresis and
- 1. The chromatography solvent is very polar as it contains alcohol, an acid and water. Based on this information, list all the polar amino acids and arrange them from most polar to least polar.8. Explain how buffer systems are important in organisms. In the human body, bicarbonate and carbonate ions work together to stabilize or buffer the pH of body fluids. What would happen to your blood if these buffering agents were removed? Use the buffers assignment to help you5. Consider two proteins with a pl of 4.5 and pl. of 7.7? Using a cation-exchange column, describe how these two proteins could be separated. Indicate which buffer you would prepare and how a salt gradient may be employed.