Unknown quiz_unknown 15(1) sadie hill

Lab Dilution problem you start with a bacterial concentration of 10,000,000 bacteria/ml Construct a dillution scheme with 5 dillutions so that you plate out 100 bacteria. Please do step by step simplified. Explain the math its been a while.  Try to write it out and not do scientific notation please. Thank You

MULTIPLE CHOICE Read the questions below carefully. There is only one correct answer for each question. 1. In a DNA sample of Escherichia coli (a bacteria), 23.6% of the nitrogenous bases are thymine. What percentage of cytosine nitrogen bases is present in this sample? a) 76.4% b) 23.6% c)26.4% d) 52.8% 2. If the adenine is located on the Z strand as shown (see attachement 3)  , then on the W strand, in the same place, we should find: a) Uracil b) Adenine c) Thymine d)Cytosine 3. Which of these deletions best represents a chromosomal deletion? (see options in attachment 3) 4. It is known that RNA is a nucleic acid responsible for the synthesis of proteins. However, there is another nucleic acid alongside RNA: DNA. What role does the latter play in relation to RNA? a) RNA is synthesized in the cell in case too large a mutation damages the DNA. b) DNA has the reproductive genetic code of all cells and passes it on to RNA. c) DNA directs the synthesis of enzymes, while RNA synthesizes…

will be focusing on genetics as a whole, but also differentiating some specifics for bacterial genetics. The area where I want you to focus for this discussion is on a gene in a microorganism that provides a characteristic you find interesting. However, I want it to be a gene that is beneficial for the microbial cell, but allows for a negative impact related to the human. For example, some strains of Staphylococcus aureus have a gene, mecA, that makes them resistant to the antibiotic methicillin. They are commonly called methicillin-resistant S. aureus or MRSA. The gene is beneficial to the bacteria because it allows for their survival even in the presence of this antibiotic but is negative for humans because it limits what antibiotics can be used for treatment. There are a large range of options here including microbe structures as well as the production of various substances so don’t limit your search.

Question:- Why does a medium containing antibiotics in transformation not distinguish all unsuccessful from successful ones? please answer quickly... it's very urgent!!!

Helping tags: Biology, microbiology . . . WILL UPVOTE, just pls help me answer the questions correctly and explain them clearly. Thanks.

Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…

DNA EXTRACTION Why do scientists isolate DNA?                                                                                                                    From what part of the subject’s body were cells collected? Give the specific purpose of each experimental step:           Lysis solution & heat:                                                                                             Salt & centrifugation:                                                                                  After centrifuging, where in the tube is the DNA? What is the purpose of adding alcohol?

No plagiarism I will double check. If u want helpful rating just do it in handwritten cursive answer 1) Is Immunoelectrophoresis one of the current analytical methods in detecting  porcine gelatin?Explain why

Experiment: Bradford protein assaygive the answers (4-5 lines) of review questions in the end. the answer should be logical and understandable and without plagiarism. avoid copy-pasting. PLEASE GIVE THE ANSWER OF 3rd AND 4th QUESTION. ITS COMPULSORY.Background information:The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assessing protein concentrations for gel electrophoresis. The method described below is for a 100 µl sample volume using 5 ml color reagent. It is sensitive to about 5 to 200 micrograms protein, depending on the dye quality. In assays using 5 ml color reagent prepared in lab, the sensitive range is closer to 5 to 100 µg protein. Scale down the volume for the "micro assay procedure," which uses 1 ml cuvettes. Protocols, including…

helping tags: Biochemistry, spectrophotometric analysis, enzyme, substrate, coenzyme Will upvote, just pls help me answer the table. Thanks.

The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?

Question:- In an early log-phase E. coli population, there are 1 x 103 cells/ml the generation time of the cells is 30 minutes. How many cells would there be 60 minutes later? a. 2X10^6b. 8X10^3c. 4X10^3d. 2X10^3e. 3X10^6