Lesson11 Actin-based motility_WORKSHEETS
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MCB*4580 Membrane Biochemistry
Lesson 11 Cytoskeleton III –Actin-based motility
Instructions:
Answer the following questions using any resources
you can obtain. When you are done, submit your answers as a Word document to the Dropbox folder
.
Contributions:
If you worked in a group, list the members of the group and their contribution(s) to these responses:
Paper #1: Loisel et al 1999
What are the three main points
of the paper? (Final paragraph Paper) List the basic components (buffers and proteins) and their concentrations in the motility medium that the authors observed Listeria
motility (Paper). Drawing on your own experience and other resources, very briefly note what each component in the solution does
(your experience + internet). The motility medium contained: What was the maximum motility rate
for Listeria
in the motility assay (Paper)? Paper #2: Ohkawa et al 2010
In Figure 1A bottom time series pictures— What did they do to the baculoviruses and the cells so they could visualize
the viruses and actin in the cells? (Methods ) What kind of cells
are these (Methods)? What organism do they come from? (Internet) What evidence from Figure 1
suggests that the motility of the viruses is due to the P78/83 protein? What evidence confirms the involvement of actin polymerization and Arp 2/3 proteins in the motility of baculoviruses? Compare the velocity
of baculoviruses to those of Listeria
or Shigella
bacteria inside living cells (Paper). THINKING QUESTION
MCB*4580 Membrane Biochemistry
You want to test the idea that the P78/83 protein from baculovirus is the only
protein required for motility of the virus in an in vitro
motility assay. What kind of object would you track and how would you ensure that you are only testing the P protein, and nothing else from baculoviruses?
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Course: SCI Biology
Unit: Unit 08: Cell Membrane
SCI Biology - Unit 08: Cell Mer
1) The process of moving a large substance into a cell by means of a vesicle.
OA.) Endocytosis
OB.) Exocytosis
Save
2) The process of moving a large substance out of a cell by means of a vesicle.
OA.) Exocytosis
R) Endorytosis
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100% water
Part D: Application
4% water
1. The paramecium (see Figure 3-1) is a single-celled organism. Go to the
web and look up this organism. Now select an answer from each blank.
H2O
(iso-, hypo-, or
nucleus
oral groove
Paramecia live in a(n)
hypertonic) environment. In this environment, the paramecium takes
in a lot of water through the process of
(osmosis, diffusion, facilitated diffusion, or active transport). In order
to get rid of this excess water, the paramecium has a specialized
organelle called the contractile vacuole that acts as a water pump
-contractile vacuole
to expel water through the process of
(osmosis, diffusion, facilitated diffusion, or active transport).
FIGURE 3-1 Paramecium.
30
Anatomy and Physiology | Laboratory Manual
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Concentration, Osmosis, and Cell Environments HW Quiz
For each of the drawings, label what kind of environment it is in, how you know this, and what is happening to the cell.
This cell is in a isotonic
v solution.
I know this because
This cell will
More water is coming in than going out
More water is going out than coming in
The same amount of water is going out and coming in
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Cell Membrane and Cell Transport WebQuest
Part I: Cell Membranes
Go to the following website: www.biology4kids.com/ files/cell_membrane.html
1. How is the cell membrane similar to a plastic bag with tiny holes?
2. What two components make up the cell membrane?
a. What are their functions?
3. What is the fluid mosaic model?
4. Sketch a section of the cell membrane, showing both phospholipids and proteins.
Label your drawing.
5. Label the diagram of the phospholipid molecule below with the following terms:
hydrophilic head, hydrophobic tail
This diagram
shows a
Head
simplified
version of a
phospholipid
molecule.
Tails
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06:33 t 42 A
Lysosome
63
Compilation of Biochemistry Laboratory Activities Prepared by
Page 4 of
Things to ponder:
1. What particular specimen was used in observing the plant cell using the virtual scope?
Specimen for animal cell?
2. The microscope used in this lab is not powerful enough to view every organelle.
Which organelle(s) can you see in the cheek cell?
3. Name two organelles that are not visible, but should be present in the cheek cell.
4. What cell structures are common to all types of cells?
5. What cell structures are present only in eukaryotic cell?
6. List the structures that are common in both plant and animal cells.
7. List the structures that make plant and animal cells different from each other.
Tabulate your answer.
Compilation of Blachemistry Laboratory Activities Prepared by
Page 5 of
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RCPS Biology CUA Unit 1/1 of 24
A student carries out an experiment to investigate the role passive transport plays in maintaining homeostasis in organisms For this investigation, the student
uses two gummy bear candies-lo represent animal cells The student records the mass of each bear Then he places one n a beaker filled with distilled water
He places the other in a beaker filled with salt water After 48 hours, he removes the bears from the beakers and records their masses again He finds that the
mass of the gummy bear in the distilled water significantly increased, while the mass of the gummy bear in the salt water significantly decreased
Which of the following is the BEST interpretation of the observations made during the investigation?
O A Distilled water was able to stick to the surface of the gummy bear when it was…
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The diagram below shows three different proteins embedded in a cell membrane, as well as the concentration gradient surrounding the cell.
high concentration
POREI
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Which of the following statements correctly states the process shown in the diagram?
O The process labeled 1 shows how cells can easily move molecules with the concentration gradient using high energy molecules.
The process labeled 2 shows how cells move molecules against the concentration gradient without needing high…
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CELL STRUCTURE AND FUNCTION – EUKARYOTES, PROKARYOTES AND VIRUSES-LABORATORY REPORT SHEET
ACTIVITY 2: CELL MEMBRANES, OSMOSIS AND DIFFUSION
Initial Volume (Vi)
(mL)
Final Volume (Vf)
(mL)
Change in Volume
(Vi – Vf)
(mL)
A
20% sucrose
20% sucrose
92.0 mL
94.0 mL
-2.0 mL
B
40% sucrose
20% sucrose
92.0 mL
92.0 mL
0.0 mL
C
20% sucrose
40% sucrose
92.0 mL
90.0 mL
2.0 mL
Treatment
Percent Change
A
-2%
B
0%
C
2%
Hypothesize which dialysis bags should gain, lose or stay the same in regards to volume. Explain.
Which solution is hypotonic, hypertonic and isotonic?
Explain what the change in volume of the dialysis tube indicated and describe what happened when the volume increased and when the volume decreased.
Explain the relationship between solute concentration and amount of free water or water available for movement.
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Question:
Explain how the strongest of the cytoskeletal filaments systems is organized?
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Abdulla Salem: Attempt 1
E) 20 kilocalories per gram of wax burned
Question 36 (2 points)
lons can travel directly from the cytoplasm of one animal cell to the cytoplasm of an
adjacent cell through
O A) gap junctions.
B) intermediate filaments.
C) plasmodesmata.
D) desmosomes.
E) tight junctions.
Question 37 (2 points)
A.
C.
В.
H.
H
H-C-N
H.
H-C-SH
H-C
H.
H.
H H
hp
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Instructions: Fill out the Venn diagram below on information regarding the parts
of eukaryotic (plant and animal) cell and prokaryotic (bacterial) cell. Overlapping
areas are cell parts that are common to both types of cells.
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e Final Part 2 (page 2 of 25)
rkanacollege.edu/mod/quiz/attempt.php?attempt=502922&cmid=3353028&page=1
Question 2
Not yet answered
Points out of 1.00
P Flag question
Which structure is the site of the packaging of proteins and lipids that may be shipped out of the
cell? It is the "“Fed-Ex" or "UPS" organelle.
Select one:
O a. Golgi apparatus
O b. tight junctions
O c. rough ER
O d. plasmodesmata
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carted: Oct 16 at 8:23am
Quiz Instructions
2 pts
Question 1
Which microscope would be best at observing the detailed internal
Tir
membrane structure of cells?
Att
34
O Transmission electron microscope
O Fluorescent microscope
O Scanning electron microscope
O Brightfield microscope
2 pts
Question 2
Which of the following does a prokarvotic cell not contain?
Desktop
82°F Sunn
99+
DELL
F12
PrtScr
Insert
F8
F9
F10
F11
F5
F6
F7
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Need help on questions 2, 3, 5, and 6, please.
Drop-down answer choices for questions 2, 3, 5, and 6.
Question (ii): No or Yes.
Question (iii): No or Yes.
Question (v):
ER: Cytosolic or Lumen.
Golgi: Cytosolic or Lumen.
secretory vesicle: Cytosolic or Lumen.
Question (vi): ER, secretory vesicle, Golgi, cytoplasm, extracellular, nucleus, or mitochondria.
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Questions
1. If the potato cells were hypotonic to the surrounding solution, the surrounding solution itself
would be what to the potato cells (hypertonic, hypotonic, or isotonic)?
2. Were the potato cells submerged in water with no salt hypertonic, hypotonic, or isotonic to the
surrounding environment?
3. Were the potato cells submerged in 5 g/100mL salt solution hypertonic, hypotonic, or isotonic to
the surrounding environment?
4.
Look at your graph. At approximately what concentration of salt solution would the potato cells
have likely been isotonic with the surrounding solution? [Tip: Estimate approximately at what
concentration there would have been no change in the average length of potato strips.]
B. Turgor and Plasmolysis
You have observed that concentration differences between cells or between a cell and its environment
can affect the movement of water across cell membranes. This can be observed using changes in volume
or size as in the previous experiment or visually, looking…
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Which electron or light microscopy technique would you use for the following? (also explain)
For visualizing the fine structure of cellular organelles and cell architecture in the cell interior using very thin cross-sections made in Epon.
To obtain sequential images of a macromolecule in order to study its activity in real-time.
To obtain an extremely clear three-dimensional image of the nucleus of a single cell.
What is the magnification of the image in a microscope using a 20x ocular and a 100x objective?
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Course X
Edio | Course x
Edio | Course x
A mitochond x Edio Course X
/1089900/lessons/1533965/variants/2428207/take/14/
A
* Practice
TEXT ANSWER
Many different microscopic organisms can be found in pond ecosystems, including the
three organisms shown in the diagrams below. The primary cellular structures in each of
these single celled organisms are labeled in the diagram. Some of the structures are com-
mon to all three organisms and other structures are not. One of the three organisms be-
low can obtain energy through photosynthesis.
a. Based on the diagrams, identify which organism is able to perform photosynthesis.
Explain your reasoning.
b. Identify the two reactants for photosynthesis.
c. At times, this photosynthetic organism can switch to being heterotrophic. Describe a
condition that would favor this organism being heterotrophic. Explain your answer.
Answer using 2 to 3 complete sentences for each question.
Organism 2
Organism 1
Cytoplasm
Chloroplast-
Cilia
Polysaccharide…
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MAIN IDEA: Eukaryotic cells have an extensive and dynamic framework called a cyto-
skeleton. The cytoskeleton is used for cell shape, internal structure, movement, and cell
division. Many cells are surrounded by and supported by a complex extracellular matrix and
are able to interact with adjacent cells and the environment via cell junctions.
5. List three common components of microtubules and microfilaments.
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A.
CELLULAR STRUCTURE AND BIOMOLECULE
1 & 2. Give two unique properties of the organelles emphasized by the endosymbiotic theory.
Identify the organelle or cellular structure based on the given reactions. (3 - 10)
3. DNA-directed DNA synthesis
4 DNA-directed RNA synthesis
5. post-translational modification of proteins
6. Glycosylation reaction
7. Lipid synthesis
8. Degradation reactions
9. Catalase activity
10. Glycolysis
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i have 4 homework questions related to cell transport. i answered them with the help of my notes but just wanted to verify if my answers are correct and if they are not then an explanation would be helpful. so can you confirm if the following bolded answers are correctly selected:
1. Two molecules that can pass easily through the plasma membrane, between phospholipids, include
a) oxygen and starch
b) salt and water
c) starch and salt
d) water and oxygen
e) starch and water
2. When comparing two solutions, the one with more dissolved solutes is said to be
a) hypotonic
b) hypertonic
c) isotonic
d) in equilibrium
3. Turgor pressure builds up in a(n)_________ cell when it is placed in a(n)_______ solution.
a) plant, hypotonic
b) plant, hypertonic
c) plant or animal, isotonic
d) animal, hypotonic
e) animal, hypertonic
4. The scent of a burning candle fills the entire room. This process is an example of
a) osmosis
b) diffusion
c) endocytosis
d) facilitated diffusion
e) none of the above
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28
Correctly complete the following sentence
Signal Recognition Particles (SRP) enable ribosomes that are making proteins to dock with the endoplasmic
reticulum. A prokaryotic cell that doesn't make this SRP is likely to
Select one alternative:
be unable to produce proteins destined for the endomembrane system or secretion
be unable to secrete proteins
function as normal
start using ribosomes free in the cytosol to produce proteins destined for the endomembrane system.
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Link each process with the correct definition. RECEPTORMEDIATED ENDOCYTOSIS PHAGOCYTOSIS PINOCYTOSIS EXOCYTOSIS 1. A cell ingests a large particle, such as a bacterial cell. 2. Receptor proteins embedded in the membrane recognize specific surface characteristics of substances. 3. A transport vesicle inside the cell approaches the plasma membrane of the cell, fuses with it, and releases its contents to the outside of the cell. 4. A vesicle containing whatever molecules are in solution outside the cell bulges inward, pinches off, and enters the cell.
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Title: Osmosis on Zucchini and Cucumber
Objective: Explain how osmosis happens in plants such as zucchini and cucumber.
PLEASE WATCH THE VIDEO LINK HERE BEFORE ANSWERING THE QUESTIONS. Thank you
Youtube link: https://youtu.be/9bPto2f_fGA
After watching it, please ANSWER THE QUESTIONS!
The Questions:
-Explain why and how did this happen to each of the slices of cucumber or zucchini. (In each Set)
-Kindly explain exactly WHY did this happen. (In each Set)
-What type of solution were present in each set up here? (In each Set)
-How did osmosis happen on each of the set ups? (In each Set)
You might be wondering what does it mean with the "(In each Set)". So the format on how you will answer the questions above is in Three sets: Set 1, Set 2 and Set 3 (you can see it in the video, MANDATORY TO WATCH IT).
FORMAT:
SET 1:
Answer the questions.
SET 2:
Answer the questions
SET 3:
Answer the questions
Thank you so much:)
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Question: The organelle is that forms the basalbodies of cilia and Flagella is
A) centriole
B) Ribosome
C) Golgi apparatus
D)peroxisome
I knew the best answer is not Ribosome and Golgi apparatus. So the what is the correct answer and rationale?
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For each of the following scenarios please describe which part of the cell theory is supported by this
evidence.
Options:
•
•
•
Cells come from preexisting cells,
cells are the basic unit of life,
all living things are made of cells
1. A botanist (plant scientists) named Schlieden studied a large variety of plant cells under the
microscope and found that all plants were made of cells.
Option 1
2. A scientist named Leeuwenhoek found that after studying a wide variety of organisms the
smallest living organism he observed was made of only one cell.
Option 1
3. A scientist named Virchow viewed cells under a microscope and found that cells would grow
and split into new cells.
Option 1
4. A zoologist (animal scientist) looked at a large amount of different animal tissues under a
microscope and found that all animals are made of cells.
Option 1
5. Robert Hooke studied cork and many other substances and found that they contained little
square boxes he called cells.
Option 1
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Transport Across the Cell Membrane Worksheet
1. For each of the following examples, state whether the solution is isotonic, hypertonic or
hypotonic and draw an arrow to indicate which way water will move.
a)
b)
100% H₂O
10% NaCl
0% NaCl
90% H₂O
83
5% salt
10% salt
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CSP1501/101/3/2
Question 1: Multiple-choice questions
1.1.
The cell membrane is important for all the following reasons except
1. it determines what substances enter and exit the cell.
2. it separates the cell's internal fluid and the surrounding extracellular fluid
3. it acts as a site for cell-to-cell interaction and recognition.
4. it is an essential site for DNA transcription.
1.2.
The function of a cell nucleus is:
hydrate production
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Mastering 7: Chapter 6
Chapter 6 Question 6
Drag the appropriate labels to their respective targets.
plasma
membrane
smooth ER
vacuole
microtubule
nucleolus
Golgi apparatus
nucleus
plasmodesma
microfilament
peroxisome
rough ER
ribosome
cell wall
nuclear pore
chloroplast mitochondrion
Submit Request Answer
Reset Help
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please choose correct letter and explain a bit
1. The strongest way by which a protein can maintain its shape is by using:a) ionic bonds
b) disulfide bonds
c) star war forces
d) van der Waals forces
2. If a cell (like an amoeba) would be capable of migrating from one place to another only with movement created from the inside of the cell, then we can say that that movement is done mainly by:
a) glycoproteins
b) feet
c) microtubules
d) actin filaments
True or False: In eukaryotic cells (such as human cells), the variety and diversity of proteins is much less than the variety and diversity of lipids.
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smi
S
ed
yn
OS
nt
de
3.
C
Nucleus
^^
mitochondria
**see instructions**
Raugh ER
k
Peroxisome
HW 9-14 DUE 9-19
Lysosome
Name
U#
Seat
What is the total cell length?
24um
What is the surface area/volume ratio?
0.2 Sum
Culgi Apparatus
2um
H
List the starting point and all the
organelles a secreted protein will
pass
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What differences are there between a free-living, single-celled organism such as a
paramecium and a single human cell such as a ciliated cell of the respiratory tract? Use cited.
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