BIOD171 Microbiology Lab Notebook
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BIO 171- Microbiology
Lab Notebook
Lab Notebook Bookmarks (click to navigate):
Lab 1 Notebook
Lab 2 Notebook
Lab 3 Notebook
Lab 4 Notebook
Lab 5 Notebook
Lab 6 Notebook
Lab 7 Notebook
Lab 8 Notebook
Lab 9 Notebook
Lab 1 Notebook
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Title: #1 Introduction to Medical Microbiology
Objective:
Cultivation of samples (growth conditions)
-
Equipment used
Identification of samples (biochemical assays)
-
Tests available Evaluation of samples (microscopy)
-
Visualization of recognition of key characteristics Procedure:
Cleaning:
-
Autoclave 125° C
Growing -
Fixed incubator 37° C
-
Shaker incubator 37° C
Visualizing -
Microscopy Storing
-
Refrigerator 4° C
Lab safety:
Never eat or drink in the lab -
contamination risk
Use personal Protective Equipment (PPE)
-
Latex or thermal gloves (application dependent)
-
Eyewear protection -
Lab coat Never leave the lab while wearing PPE
-
Public hallway -
Bathroom
-
Cafeteria Notes:
Additional helpful information placed here
Results:
A summary of the final outcome of the experiment should be here
Lab 2 Notebook
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Title: Basics of Microscopy Objective: To become familiar with the basic components of a light microscope as well as how to load a sample for viewing.
Procedure:
1.
Review parts and components 2.
Load sample slide into microscope 3.
Select magnification 4.
Make necessary adjustments to optimize sample visualization Notes:
Parts of microscope:
1.
Eye pieces (Ocular lens): Can be adjusted to fit the width of your eyes. Should be able to use both eyes but you should see a single circle within the viewfinder. 2.
Arm/neck: When moving the microscope keep one hand on the neck and another underneath the base. 3.
Objectives: Lenses that provide part of the magnification to the microscope. 4 different objectives with revolving nose. Shortest objective 4x magnification, others include 10x, 40x, and 100x. 4.
Stage: flat surface where you place the sample. Includes a clamp holder and stage clips
to hold the sample in place. 5.
Focus knobs: Outside ring is called course adjustment used when you want to make large steps in changes of your focus. Inner knob is called fine focus used when you can see the object but need to focus more to see details. 6.
Iris or Diaphragm: Controls how much light is let into the microscope and ultimately into the eyepiece.
7.
Base: where the microscope sets. Always make sure the base is steady.
8.
Glass slide: Located on the microscope stage. 9.
Stage guide: There are two knobs. One knob controls vertical axis to move forward and backwards, another knob to move left and right. Visualization: -
Types of objectives: Dry vs Oil
-
Intensity of light source: Too bright= saturation, Too dark= low visibility -
Power of objective x Power of eyepiece= Total magnification: If a cell is 15 mm in diameter, using a 40x objective and 5x eyepiece the cell will appear to the eye 200 times larger or around 3000mm in diameter. Results: Magnification = object x eyepiece
Lab 3 Notebook
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Title: Mounting Techniques Objective: Microscopic examination of bacterial samples through various staining techniques.
Identify color and shape of given samples. Procedure:
Dry Mount:
1.
Clean slide (70% ethanol)
2.
Circle area on slide for easy location of specimen (optional)
3.
Apply organism to slide: If from culture, use sterile loop to spread onto slide. If from plate, use sterile loop to pick colonies and mix with a drop of distilled water.
4.
Air dry at room temperature until all moisture has evaporated. Wet mount:
1.
Clean slide (70% ethanol)
2.
Circle area on slide with a wax/hydrophobic pen 3.
Apply organism to slide: If from culture, use sterile loop to spread onto slide. If from plate, use sterile loop to pick colonies and mix with a drop of distilled water.
4.
View under microscope: Wet mount is ideal for viewing the motility of an organism. Do not dry out. Gram Staining:
1.
Clean slide (70% ethanol)
2.
Apply organism to slide: Use sterile loop to spread ~ 1-3 drops onto slide. Spread into a thin film 3.
Allow to air dry
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Related Questions
MICROBIOLOGY: Microscopic Morphology of Microbes
Write your introduction (This includes principles, significance of the study, objectives of the experiment and how the objectives were achieved. This part must also be in the passive voice and past tense. Introduction must be short but packed with relevant content).
another:
What is the advantage of the Gram stain over the simple stain?
What is the theory about the mechanism of the Gram-stain reaction?
arrow_forward
Subject: Microbiology
** Pls answer all items. Thank you
arrow_forward
microbial sensitivity lab: Why should you avoid looking directly into the ultraviolet (UV) light?
arrow_forward
MICROBE PROFILING
Instructions:
Choose any microorganism from the different groups of cellular and acellular and fill in the information required for your chosen microbe for profiling.
1. Scientific Name and common name of the microbe:
2. Importance:
3. Cellular or Acellular?
4. If cellular, eukaryote or Prokaryote?
5. If cellular, which Kingdom?
6. General Morphology (describe cell types, parts of cells, size, etc.):
7. Where are they commonly found?
8. Source of nutrition:
10. Does it cause human infection? (Yes or No); What disease?
11. If they cause diseases, how can we prevent the infection?
12. Representative photo
13. References:
arrow_forward
INSTRUCTION:
Answer the question properly
Do not copy in Google, plagiarize checker will be used.
QUESTION:
Tabulate the main differences between Gram-positive and Gram-negative bacteria.
arrow_forward
Hello, please read the attached Microbiology question and answer the question correctly. Please aim for about 3-4 sentences max (enough to fit within the lines given). Write in complete sentences (No Bullet Points).
*If you correctly answer the question, I will provide a Thumbs Up to you.
Thank you.
arrow_forward
Question:-
Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need).
Introduction
Discussion
References
arrow_forward
Hello, please read the attached Microbiology question and answer the question correctly. Please aim for about 3-4 sentences max (enough to fit within the lines given)
*If you correctly answer the question, I will provide a Thumbs Up to you.
Thank you.
arrow_forward
Give the uses/functions and images of each apparatuses.
Basic Laboratory Equipment
Uses/Functions
Picture
1. Microscope
2. Colony Counter
3. Autoclave
4. Microbiology incubator
5. Drying oven
6. Refrigerator (microbiology)
7. Bunsen burner/alcohol lamp
8. Candle jar
9. Anaerobic jar
10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet
11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)
12. Petri dish
13. Culture tubes
14. Hanging drop slide
15. Durham’s tube
16. Staining rack
17. Thermostatically controlled water bath
18. Inoculating loop
19. Inoculating needle
20. Vials
arrow_forward
Activity 2. Media Used in Isolating Coliforms
You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are
asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these
bacteria. Include pictures and references.
Bacteria
Enrichment
(Broth/Agar)
Presumptive test
(Broth/Agar)
Isolation Media
(Broth/Agar)
E. coli
Salmonella
Shigella
1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water
station? Explain.
2. What is the difference between nutrient broth and nutrient agar?
3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria?
Conclusion about the process of isolating bacteria
arrow_forward
Give the uses/functions and images of each apparatuses.
Basic Laboratory Equipment
Uses/Functions
Picture
1. Microscope
2. Colony Counter
3. Autoclave
4. Microbiology incubator
5. Drying oven
arrow_forward
microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?
arrow_forward
Instruction: answers must be in numbers.
You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have. However, your microbiology instructor wants to know how many bacteria per milliliter (bacteria/mL) are on your plate. And as you have remembered during the first day of inoculating this bacteria you used a imL aliquots sample of a 1:10,000 dilution, and the total colony you counted on this plate is 170. How many bacterial per milliliters would that be?
arrow_forward
Hello, please read the attached Microbiology question and answer the question and its two parts correctly. Please have your answer fit within the two lines given for each part (Please do not give me a paragraph as an answer).
*If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.
arrow_forward
Name:
Chemotaxis Lab Worksheet
1. What is a model organism and why is it important to scientific studies? List two common model
organisms, one for prokaryotic studies and one for eukaryotic studies.
arrow_forward
Please match the fields of microbiology with the statements that most accurately describe them to test your understanding of the
primary areas of study within microbiology.
1. The field dedicated to monitoring and controlling the spread of disease within a population
(Click to select)
2. The field which uses microbes to produce specific desired products (Click to select)
3. The field which studies the protective reactions to microbial infections, examples include blood testing and vaccination
(Click to select)
4. The field which manipulates DNA of an organism to create a new organism with a desired trait
(Click to select)
5. The field which studies the relationships between microbes and domestic plants and animals
(Click to select)
6. The field which is concerned with food-borne diseases as well as food and beverage production
(Click to select)
arrow_forward
site your reference:)
What are the basic concepts and precautions to be observed in specimen collection for Microbiological examination?
Enumerate specific examples of clinical specimens collected for microbiological examination. Give 2 specific examples of bacteria isolated from clinical samples.
What is a transport medium; give specific examples and state their purpose.
arrow_forward
Hello, please read the attached Microbiology question and answer the question and its parts correctly. Please have your answer fit within the one line given for each part (Do not give me long answers, just tell me what it detects).
*If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.
arrow_forward
Give the uses/functions and images of each apparatuses.
Basic Laboratory Equipment
Uses/Functions
Picture
1. Microscope
2. Colony Counter
3. Autoclave
4. Microbiology incubator
5. Drying oven
6. Refrigerator (microbiology)
7. Bunsen burner/alcohol lamp
8. Candle jar
9. Anaerobic jar
10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet
arrow_forward
Worksheet Table 1. Special Staining Methods
Staining Technique
Stain
Cellular structure/
Bacteria
Example : Albert
Malachite
green and
Metachromatic granules
toluidine blue
Dorner
Dyar
Feulgen
Fontana-Tribondeau
Gray
Leifson
Levaditi's
Neisser
Nigrosin
Schaeffer-Fulton
arrow_forward
Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)?
Staining (to increase magnification).
Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation).
Add immersion oil (to increase resolution).
Add a cover slip (to reduce contamination).
The viewing chamber needs to be flushed of air (to create a vacuum).
arrow_forward
INSTRUCTIONS:
Please do not copy here in Bartleby or in Google.
QUESTIONS :
1. Why is it importance to heat the lid of the petri pate where you will grow the microorganism? Explain.
arrow_forward
Give the uses/functions and images of each apparatuses.
Basic Laboratory Equipment
Uses/Functions
Picture
6. Refrigerator (microbiology)
7. Bunsen burner/alcohol lamp
8. Candle jar
9. Anaerobic jar
10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet
11. Bacteriologic filters (Seitz, Chamberlain, Berkfield)
12. Petri dish
13. Culture tubes
14. Hanging drop slide
15. Durham’s tube
arrow_forward
can somebody please help me to write Introduction: for unknown bacteria of microbiology
This section introduces the reader to the study and why the study was done. the importance of learning about potential pathogenic agents and the importance of determining the unknown identity of these organisms in a clinical setting. around 5-8 sentences.
arrow_forward
G Search results - Ifortun1@email.essex.edu - Esse
cmiguel@gmail.com-Gmail
Open with Google Docs
C.
D
Figure 9.2
Using Figure 9.2, match the following:
42) I band.
42)
43) H zone.
43)
44) A band.
44)
45) Z disc.
45)
46) M line.
46)
Page
8 | 8
+
arrow_forward
Helping tags: Biology, Food Microbiology, Spore formers, sulfide spoilage spores
25 g of sugar sample was obtained from the production line for analysis of raw ingredients. It was diluted to 250 ml and was heated at 100 deg Celsius for 5 minutes. After cooling, 20 ml of the sample solution was divided equally among 6 tubes of sulfite agar. After incubation, the following counts were obtained: 35, 26, 29, 32, 34, and 31. Compute for the number of sulfide spoilage spores and express the count per 50 g of sugar.
.
.
.
WILL UPVOTE, just pls help me answer the question and show COMPLETE solution.
arrow_forward
Hello, please read the attached Microbiology question and answer the question and its two parts correctly. Please have your answer fit within the two lines given for each part (Do not give me long answers).
*If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.
arrow_forward
microbial sensitivity lab: Why is the method of testing chemical sensitivity to disinfectants you performed considered somewhat inaccurate?
arrow_forward
Consider the biotech lab. In the plate images below, what does P+ mean? Write
your answer below. Be clear and specific.
Bacterial Plating Conditions
P-P+
P-IP+
P+
LB plate
LB/amp plate
LB/amp/ara plate
arrow_forward
INSTRUCTION:
Answer the question properly
Do not copy in Google, plagiarize checker will be used.
QUESTION:
Discuss the various theories on the Gram stain.
arrow_forward
Hello, please read the attached Microbiology question and answer the question and its two parts correctly. Please determine the dilution factor / final dilution based on the question. You do not have to give me a long explanation as to how you got the answers.
*If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.
arrow_forward
Greetings :) Based on the results of using Hanging-drop method in Figure 2, what microorganisms can you find? Kindly state or identify all microbes you can see, and briefly described each of them. Thank you so much!
arrow_forward
Support your answer using computation-based judgment. Encircle/box your final computed answer in your solutions along with a declaration of TRUE or FALSE for each given situation.
UHT-pasteurized milk sample X which was spread-plated in quadruplicates yielded the following bacterial colony counts: 23, 20, 35 and 41 CFU/plate at a dilution factor of 100. Therefore, the milk sample will pass laboratory standards if the quality control laboratory sets the total microbial limit as: “not to exceed more than 30,000 CFU/mL of milk sample”.
arrow_forward
Please use this submission link to submit your flowcharts. You will need to submit a total of three flowcharts, one for each of your unknown microorganisms (gram positive rod, gram positive coccus, and gram negative rod). Please make sure that you only use the allowed tests: and microorganisms to complete your flowchart. You must identify each of the allowed organisms to a tip on the flowchart. Each organism must take no more than 3 total tests to completely identify
POSSIBLE MICROBES & ALLOWED TESTS
MICROBE
Gram rxn/shape
Catalase Activity
Mannitol/High Salt (growth/ferm)
H2S prod.
Lactose ferm./gas
EMB (growth/pigment)
MR /VP
Indole
Citrate
Colony Color
Enterobacter aerogenes
negative rod
-
+ / +
growth/pigment (pink fisheye)
- / +
-
+
opaque
Escherichia coli
negative rod
-
+ / +
growth/pigment (metallic green sheen)
+ / -
+
-
opaque…
arrow_forward
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- Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion Referencesarrow_forwardHello, please read the attached Microbiology question and answer the question correctly. Please aim for about 3-4 sentences max (enough to fit within the lines given) *If you correctly answer the question, I will provide a Thumbs Up to you. Thank you.arrow_forwardGive the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. Vialsarrow_forward
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