8L.Part I You repeat your kinetic assay for three additional substrate concentrations and determine the initial velocities using the same approach as above. These values,originally obtained in units of mAbs/s, are then converted into units of nmol/min. A summary of all of measured initial velocities for each amoxicillin concentrationis provided in the table.102550100200300+B[Substrate](µM)Draw a graph of initial velocity versus substrate concentration. The labels for the x and y axes are intentionally left blank. Determine the dependent and independentvariables before graphing. Label the plot direct.No elements selected3002752502252001751501251007550254.810.918.829.541.147.3YInitial velocity(nmol/min)EDD?I25 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400

Michaelis-Menten Kinetics is one of the simplest approaches to enzyme kinetics.The basic equation is…

Answered: You repeat your kinetic assay for three…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?
Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength.
Calculate the Activity of an amylase enzyme which is diluted 1:100 times with phosphate buffer and incubated for 10 minutes at 37 degree Celsius. Given are the amount of maltose [mg] = 5.05 and the volume of enzyme used for the assay as 0.5ml.
Paclitaxel is used to treat ovarian cancer by infusion at a concentration of 0.6 mg/mL over 24 hours. A dose of 135 mg/m² has been prescribed for Sero. Sero is 1.06 m tall and weighs 92 kg. Paclitaxel comes in vials of 150 mg/25 mL. Calculate the volume of 5%w/v glucose solution that would be required to dilute the concentrated solution to prepare an paclitaxel solution of the required strength. Answer: You did not give the correct unit. The correct answer is: 333 mL X
Various concentrations of recombinant human insulin were prepared for use standards for an HPLC method. To verify the prepared concentrations, the samples were analyzed by measuring the absorbance at 280 nm in a short path length (5 mm) cuvette. The molar absorption coefficient for human insulin is approximately 5.875 x 10³ M-¹cm-¹. a. Calculate the extinction coefficient in mL mg-¹cm-¹. b. Calculate the concentrations of the following human insulin standards if the measured absorbances and dilutions used are: Standard 1 Standard 2 Abs. (at 280 nm) of Diluted Sample 0.305 0.685 Dilution 145.0 µL sample, 25.0 μL buffer 130.0 µL sample, 40.0 μL buffer
A purified protein sample was used in a reaction, resulting in an activity of 696.7 nmol min-1. The reaction volume was 145.0 µL and the final volume before loading the plate was 1,050 µL. The total reaction time was 4.25 min. The amount of protein used in the reaction was 4.270 µg. Calculate the specific activity of the sample (in nmol min-1 µg-1).
after a 5 g onion sample is divided into two equal parts, heat treatment to one of these partsit is being implemented. Then both heat-treated and non-treated parts are 10 times their weightit is ground in an environment containing as much buffer and the determination of pyruvate in the supernatants obtainedit's being done.a) As a result of measurements taken at 520 nm; absorbance value for the heat-treated sample;it was found that the absorbance value in the sample that was not heat treated was 0.123 and the absorbance value in the sample that was not heat treated was 0.520.The equation of the calibration accuracy for pyruvate is that y = 0.1367x – 0.001 (nmol/mL)according to the onion sample; a) independent of alinase activity; b) dependent on alinase activityand c) calculate the total pyruvate amounts in terms of nmol/g of onion.b) How is there a relationship between the activity of the enzyme allinase in onions and the amount of pyruvate?Dec,please explain.
You plot a reaction progress curve and find the equation of the line to be y = -0.0961x + 0.7142. Calculate the U, U/μL and U/mL of lysozyme activity in this fraction. The lysozyme activity is done identical to what is indicated in the lab manual.
Cimetidine is used in the treatment and management of gastric ulcers and gastroesophageal reflux disease. In the case of an active ulcer, cimetidine can be administered continuous by IV infusion at a dose rate of 37.5 mg/hr. If the concentration of the cimetidine solution is 6 mg/ml, then how many milliliters must be available for a 4-hr infusion?Misoprostol is used to prevent NSAID-induced gastric ulcers. A daily dose consists of two tablets administered four times each day. What is the size of one day’s dose if each tablet contains 100 mcg of misoprostol?
This graph represents a standard curve from a BCA assay for protein concentration. If your BCA reaction with your unknown protein has an absorbance of 0.2, approximately what concentration of protein is in your unknown sample? Absorbance (562nm) 0.5 0.45 3 0.35 0.3. 0.25 0.15. 0.1- 0.05 ~0.1 μg/ml ~4.5 μg/ul less that 0.005 | 0.2 μg/μ. [Protein (BSA)) (pg/ml)
Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH Pls show sol'ns 1. Given the following reagents, give the moles of each component (acid & base).2. What are the mass/volume of the components needed to prepare the buffer? 3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?
Determine a molecular cause that allows explaining the BIOCHEMICAL alterations found in the following patient; Determine their estimated 10-year cardiovascular risk. A 37-year-old patient, a woman who has smoked for 10 years, consulted for malaise, headache, nausea, and fatigue. The medical examination found tendon xanthomas, skin xanthomas, signs of atherosclerosis. Laboratory test Glycemia 90 mg/dL, total cholesterol 560 mg//dL, TAG 230 mg/dL, cHDL 33 mg/dl.

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