what is the is the dilution factor when you add 2 mL ssample of 8 mL diluent?
Q: You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution;…
A: It is basically done for counting the microorganisms like viruses, bacteria etc from an unknown…
Q: You were instructed to add 0.2 ml of kombucha to 9.8 ml of fresh tea, but instead added 2.0 ml of…
A: Dilution is defined as the ratio of stock volume to the final volume. Here, final volume is the sum…
Q: Is the time required to Elute substance A, greater if N=1000 or N=2000?
A: N is number of theoretical plates in column Retention time (RT) is a measure of the time taken for a…
Q: What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?
A: Biopolymers are polymers, mainly polysaccharides, which are produced by microbes. They are mainly…
Q: What is the purpose of doing the silver nitrate test? Write the chemical equation involved here
A: Introduction: Silver nitrate is an inorganic compound and its formula is AgNO3 with a molar mass…
Q: Kovac's reagent is added to detect indole. True or False?
A: True
Q: What volume of 10X TBE buffer should you use to make 100mL of 1X TBE buffer? 10mL 1mL 0.1mL 100mL
A: TBE buffer stands for Tris-Borate-EDTA buffer. It is used for both agarose and polyacrylamide gel…
Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…
A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…
Q: Why is the annealing temperature different between samples? How is this computed?
A: Introduction Polymerase chain reaction or PCR laboratory technique by which millions to billions of…
Q: Why is there a need to filter the alkaline solution after adding the Barium chloride solution?
A: In an aqueous solution, barium chloride acts as a simple salt. However when dissolved in water, it…
Q: Calculate pNF concentration in each cuvette using Beer-Lambert’s Law. ε = 18,000 M-1 cm-1; b =…
A: Spectroscopy is the interaction between electromagnetic radiation and matter. If transmittance T…
Q: If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the…
A: Dilute formalin is used as a fixative in histological slides. The standard stock solution of…
Q: Using 25 µL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ul
A: Firstly, we convert the 25ul into ml . i.e. 25 ul = 0.03 ml Then, there will be the multiplication…
Q: what is the effect and rationale when three milliliters instead of 1 mL was placed in the Petri dish…
A: Pour-plate method is usually the method of choice for counting the number of colony-forming bacteria…
Q: 50 mL of 5mM NaCl solution from 1 M NaCl solution
A: Dilution is the process by which we decrease the concentration of a solution. This process is vital…
Q: Why did the blank used in experimental procedures contain substrate, buffer and water?
A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…
Q: When conducting a flame test for sodium iodide, is the result positive or negative?
A: An ionic compound is one that is formed between a metal or metalloid and a non-metal. A Flame test…
Q: You receive an order for 750 mL of 27% dextrose solution. You have available D50W, 1000 mL. How many…
A: Given, D50W means 50% dextrose solution, 100 ml 750 ml of 27% dextrose is desired Sterile water
Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…
A: Given data: Each plate contains- LB agar=20ml Ampicillin= 150 micrograms per ml. Amount of…
Q: What is the fluid thioglycollate medium? Please explain the testing and its results and the…
A: Microbiology laboratory uses various kinds of culture media to identify the biochemical…
Q: What is the concentration of a 50% solution after completing three 1:10 serial dilutions?
A: Serial dilution is the process that reduces the concentration of a solution in an organised manner.…
Q: You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing…
A: In biochemistry, a stock solution is a high volume of a standard reagent. They're utilized for a…
Q: To determine the cfu/ml of a pondwater sample, you perform 1/10, 1/100, 1/1000 and 1/10,000 serial…
A: Serial dilution is a technique which is adopted to dilute the highly concentrated microbial culture…
Q: Ninhydrin test for amino acids or protein requires that sample test solution should be at initially…
A: Proteins are one of the essential macronutrients for a human body. The building blocks of proteins…
Q: What is the percentage of ethyl alcohol by volume in 750 ml grape wine with 93.8 ml ethyl alcohol?
A: The wines are produced from fruit juices that are rich in glucose. The juice is allowed to ferment…
Q: Will the given lipid test positive in the Ninhydrin test?
A: Ninhydrin test is used to check whether a given sample contains amines or alpha-amino acids. The…
Q: You have a 6 M solution of stock A. You do the following serial dilutions: Add 1 ml of stock A to…
A: Dilution is calculated by stock volume to the final volume. Solution = Stock volume/ (stock volume+…
Q: How much agarose powder (g) would you use to make a 50 ml, 1.4% agarose gel?
A: Introduction:- Agarose powder is a linear polysaccharide that forms a gel when it is mixed with…
Q: Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______…
A: Dilution is the technique of lowering the concentration of a solute in a solution by merely adding…
Q: When counting WBCS, what is the best diluent? O Turk's solution O 0.85% NaCI O 70% alcohol O…
A: In the immune system, lymphocytes refer to the white blood cells that play an important role in the…
Q: If a patient is prescribed 300 mg of potassium chloride, what is the corresponding milliequivalent?
A: Drug calculation is a important aspect in calculating the dose based on the BMI ,BSA ,age and body…
Q: How would you classify a patient with the following laboratory values? Cr 0.9 mg/dl, BUN 51 mg/dl.,…
A: In azotemia, BUN, creatinine in blood and other secondary waste products are increased in body.…
Q: Using 25 uL of a patient's serum, how much diluent would be needed to create a 1:2 dilution? ______…
A: Blood is found in the human body's capillaries/veins/arteries, which are blood vessels that flow…
Q: Table of BSA solution absorbance of concentration at 595nm. BSA Concentration (ug/ml) OD595nm Group…
A: It's the table based on BSA solution absorbance of concentration of 595nm There's taken OD at 595…
Q: How do you make 70 ml of a 60% solution??
A: The making of solutions of different concentrations can be achieved with the help of principle…
Q: Why does Alka-Seltzer foam and bubble when dissolved in water? Which ingredient is the antacid?
A: Alka- Seltzar is a type of medicine which is used to cure indigestion, stomach pain or upset…
Q: If a pharmacist added each of the following to 22-g packages of 2% mupirocin ointment, what would be…
A: Mupirocin is an antibiotic that prevents bacteria from growing on your skin. Mupirocin topical is…
Q: Why do we need to place the lipid samples under direct heat after adding ethanol in a solubility…
A: The solubility test determines the presence of all lipids as a preliminary test.
Q: what is the milliequivalent weight of sodium lauryl sulfate
A: Sodium lauryl sulfate (SLS), or sodium dodecyl sulfate, is an anionic surfactant. It is commonly…
Q: if you perform a 1:5 dilution of a 1 M solution , how many parts of the 1 M solution do you use, and…
A: Dilution factor describes ratio of aliquot volume to final volume. It is a notation often used in…
Q: What two procedures should you routinely carry out to verify the purity of your working stock?
A: The 2 procedures are shown below.
Q: how many grams of sr(no2)2 are needed to make 200 ml of 0.300M solution of sr(no2)2
A: Molarity is defined as the number of moles dissolved per 1000 ml of solution.
Q: The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula…
A: Original cell density (OCD) can be found using the number of colonies of colony-forming units,…
Q: An unknown sample was titrated with CH3COOH. After making the titration curve, no steep change was…
A: CH3COOH is a weak acid hence it will not dissociate completely in the aqueous solution to form…
Q: What will happen if the water completely evaporates and was not replaced immediately? How will this…
A: The unknown concentration of sugar in a sample can be estimated by titration of the sugar sample…
Q: What is the difference between a tincture and an aqueous solution?
A: Given: Explain the difference between a tincture and an aqueous solution
Q: What does “1 Millimolar” (1mM) mean
A: Milli is a suffix in measurement meaning 1/1000 or 10^-3.
Q: What concentration of ethanol is considered optimum for disinfectant? Why not 100%?
A: Disinfectant refers to the chemical agents that destroy or inactivate microorganisms on an inert…
Q: Rocephin (ceftriaxone sodium) 370 mg IM stat.The label on the ceftriaxone states to reconstitute the…
A: Question is related to drug calculation. Ordered dose = injection Rocephin ( ceftrioxone sodium )…
what is the is the dilution factor when you add 2 mL ssample of 8 mL diluent?
Learn your way
Includes step-by-step video
Step by step
Solved in 2 steps
- If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?
- You have on hand Atarax 10mg / 5 * ml The order is for Atarax 25mg. How many ml will you give?a)Determine the amount of X (in gram) in order to prepare 100 mL of 50 mM stock solution of X. Show your work. (MW of X= 225) b)Determine the volume required (mL) from the stock solution X in (i) to prepare 500 mL of MS medium containing 5 mg/L X. Show your work.A 510 mL IV bag contains a 25% dextrose solution. How much of the original solution must be replaced with a 65% dextrose solution to increase the original concentration to 35%?
- Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.How many mL of the 1 M glucose stock solution do you need to prepare 100 mL of a 1 mM glucose solution?
- You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.To make 100 ml 0.3% NP-40 solution for FISH post washing, how many ul 100% NP-40 solution is needed?As a part of a clinical study, a pharmacist is asked to prepare a modification of a standard 22 g package of a 2% mupirocin ointment by adding the needed quantity of mupirocin powder to prepare a 3% w/w mupirocin ointment. How many mil- ligrams of mupirocin powder are required?