Using the data below, please create a calibration graph and determine the concentration of the unknown sample. Standard #1 Standard #2 Standard #3 Unknown sample Concentration 1.5 x 103 M 2.0 x 10° M 2.5 x 10 M ? Absorbance 0.61 0.80 0.98 0.72 a) 1.6 x 10 M b) 1.8 x 10 M c) 2.1 x 103 M d) 2.2 x 103 M
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- From the following data, calculate the concentration of the analyte in the sample read at 700 nm: Absorbance of unknown sample = 0.807 Absorbance of a 130 mg/dl standard = 0.234 Do not answer in image format. Maintain accuracy and quality in your answer. Answer completely.You begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN2+. To do so, you add 2.422 mL of 0.200 M Fe(NO3)3 to a cuvette and then directly add 215 µL of 0.001 M KSCN. What is the resulting concentration of FeSCN2*, assuming complete conversion of SCN' to FeSCN2+? Enter your answer in units of mM to four digits after the decimal.Table II Cuvette # Concentration Absorbance 0.50 M 1 1.049 2 0.40 M 0.926 3 0.20 M 0.740 0.298 4 0.10 M 5 0.05 M 0.202 Unknown # 9 0.427 Unknown # 10 7 0.646 Blank (DI water) 0 1. If Beer's Law is valid, the value of "E" should be constant. Use A = E*C*L or E= A/(E*L) and the data from Table II to calculate values of E for the five solutions below The value of L is normally 1 cm so E = A/C in this experiment. Table III Solution # Calculated Values of E 1 2 3 4 5
- Construct a calibration curve based on the following data: Concentration Fe 0.100 0.500 2.00 4.00 7.00 (ppm) Absorbance 0.0122 0.0608 0.2322 0.455 0.5858 Use LINEST to find the slope and intercept and their uncertainties. Enter your answer as a number with no units to three significant figures. 0.085 0.037 b m 0.100 0.500 Sm Sb r2 0.958 0.037 SyYou begin preparation of the calibration curve to measure absorbance vs concentration of FeSCN²+. To do so, you add 1.58 mL of 0.200 M Fe(NO3)3 to a cuvette and then directly add 316 µL of 0.001 M KSCN. What is the resulting concentration of FeSCN2+, assuming complete conversion of SCN´¯ to FeSCN²+´ Enter your answer in units of mM to four digits after the decimal.The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1
- The following is a standard solution of phosphorus (P) data measured by a spectrophotometer. ppm P Absorbansi 2150 0.053 4260 0.110 6400 0.173 8520 0.220 A sample of 2.1 g Na2HPO4 is dissolved and diluted to a volume of 100 mL. The absorbance of the diluted solution was measured with a spectrophotometer and obtained an absorbance of 0.128. What is the percentage of phosphorus in the sample?Ben ran through the experiment and found the best-fit line listed below for his standard calibration curve of absorbance vs concentration in M. y = 3214.0x + 0.0076 Ben then prepared a solution that had 5.00 mL 0.002 M iron(III) nitrate in 1 M nitric acid, 3.00 mL 0.002 M potassium thiocyanate, and 2.00 mL DI water. If the absorbance for the solution was 0.436, calculate the equilibrium concentration of iron(III) thiocyanate in the solution. Report your answer in mM with three places after the decimal.The absorbance of a cationic iron(II) sample solution was measured in a spectrophotometer, but the instrument returned an error because the absorbance was too high. The sample was then diluted by using a pipette to take 100.0 μLμL of the sample and injecting it into a cuvette already containing 2.00 mLmL of water (total volume is 2.00 mLmL + 100.0 μLμL). The absorbance value of the diluted solution corresponded to a concentration of 7.71×10−6 M M . What was the concentration of the original solution? Express the concentration to three significant figures with the appropriate units.
- ■ True or False: in a TOF analyzer, a fragment of low mass reaches the detector first ▪ In GCMS when we know what analytes we are measuring we use not know what analytes are in the sample we use than scan. ▪ A) Scan; SIM ■ B)SIM; scan • C) SIM; SIM mode. When we do mode. SIM allows greater sensitivity2 What methods are used to sep X G In Part Il of experiment 5, a 1:1: X + u/ultra/courses/_393452_1/cl/outline * Question Completion Status: QUESTION 3 After you complete the second step, you will be left with a clear colorless liquid. What should be done to the liquid to finish the separation? O Heat the mixture to evaporate the liquid O Add a base to neutralize the solution. O Nothing - you are done since you have a pure, clear, colorless substance O Add the residue back to the solution and dry. QUESTION 4 In Part II of experiment 5, a 1:1:1 mixture of sand, salt, and salicylic acid must be separated. Order the following steps necessary to separate this mixture into three pure, dry solids. Filter the liquid leaving a filtrate with just salt dissolved and a residue of sand. v Add hot water to the residue obtained in two steps prior to this one. Filter the liquid leaving a filtrate with just salicylic acid dissolved and a residue of salt and sand. v Heat the filtrate obtained in two…4. You prepare several dilutions of an unknown compound. You measure the absorbance of each solution at 340 nm using a 1 cm cuvette (your results are listed in the table below). What is the extinction coefficient (in (M•cm)') of the compound? (Hint: assume that each of the individual values contains some degree of experimental error.) Concentration(uM) Absorbance at 340 nm 10.0 0.011 20.0 0.023 40.0 0.066 80.0 0.119 160.0 0.189