) Use the space below and draw out the agarose gel described above. Use your drawing to answer the next questions. b) How many bands of DNA are there in lane 3? c) How many bands of DNA are there in lane 5? d) There would be 2 bands of DNA in lane 4. How big are they? e) Which lane contains the largest band of DNA? How big is it?

) Use the space below and draw out the agarose gel described above. Use your drawing to answer the next questions. b) How many bands of DNA are there in lane 3? c) How many bands of DNA are there in lane 5? d) There would be 2 bands of DNA in lane 4. How big are they? e) Which lane contains the largest band of DNA? How big is it?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.3: Microhematocrit

Problem 3RQ

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Part 4. Putting It Together

1) Consider the diagram below as well as the given information.

This diagram represents a piece of circular DNA which was cut in 4 separate reactions (4 different test tubes, each with some of this DNA in it). One digest was done with AvaI, another with ClaI, a third with EcoRV, and a fourth with ScaI. The locations of the recognition sequences for each restriction enzyme are shown along with the location of that site in bp along the circle (it goes clockwise from position 1). You run an agarose gel with a molecular weight marker in the first lane, the AvaI digest in lane 2, the ClaI digest in lane 3, the EcoRV digest in lane 4, and the ScaI digest in lane 5.

a) Use the space below and draw out the agarose gel described above. Use your drawing to answer the next questions.
b) How many bands of DNA are there in lane 3?

c) How many bands of DNA are there in lane 5?

d) There would be 2 bands of DNA in lane 4. How big are they?

e) Which lane contains the largest band of DNA? How big is it?

EcoRV (16000)
AvaI (14000)
Scal (12000)
ClaI (11000)-
Scal (1)
Ava I (1000)
(18000 bp)
Probe
ClaI (2500)
Scal (6000)
ClaI (7000)
EcoRV (8000)

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