The isoelectric point, pI, of the protein trypsin is 10.5, while that of horse liver alcohol dehydrogenase is 6.8. What is the net charge of trypsin at pH 5.1 ?[ What is the net charge of horse liver alcohol dehydrogenase at pH 7.5 ?[ The isoelectric point of cysteine is 5.07; threonine, 5.6. During paper electrophoresis at pH 4.3, toward which electrode does cysteine migrate? During paper electrophoresis at pH 4.5, toward which electrode does threonine migrate?

The isoelectric point, pI, of the protein trypsin is 10.5, while that of horse liver alcohol dehydrogenase is 6.8. What is the net charge of trypsin at pH 5.1 ?[ What is the net charge of horse liver alcohol dehydrogenase at pH 7.5 ?[ The isoelectric point of cysteine is 5.07; threonine, 5.6. During paper electrophoresis at pH 4.3, toward which electrode does cysteine migrate? During paper electrophoresis at pH 4.5, toward which electrode does threonine migrate?

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation

Section: Chapter Questions

Problem 30.3QAP

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The isoelectric point, pI, of the protein hexokinase P-I is 5.25, while that of alkaline phosphatase is 4.5 What is the net charge of hexokinase P-I at pH 5.1 ?| What is the net charge of alkaline phosphatase at pH 2.1 ?[ The isoelectric point of glycine is 5.97 ; threonine, 5.6. During paper electrophoresis at pH 7.1, toward which electrode does glycine migrate? During paper electrophoresis at pH 4.5, toward which electrode does threonine migrate?
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The amino acid serine has two pKa values, one for its carbonyl group (pKa = 2.21) and one for its NH3+ group (pKa = 9.15).These pKa values help to determine the isoelectric pH (also known as the isoelectric point, pI). The pI is the pH at which the substance has a charge of zero. It affects how the amino acid moves in gels of different pH values during electrophoresis.
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4) In the lab you are extracting and enzyme called catalase from a potato and want to use this enzyme to speed up a reaction in the lab. The potato usually grows in soil pH of 5.5 and a temperature of 18 °C. You extract the enzyme and run a reaction with it in the following conditions. pH 9.5 and temperature = 20 °C. What can be said about the enzymes activity? If activity is low how would you correct this issue? Please explain in detail and in your own words.
The isoelectric point, pl, of the protein trypsin is 10.5, while that of glyceraldehyde-3-phosphate dehydrogenase is 6.55. What is the net charge of trypsin at pH 5.1 ?[ What is the net charge of glyceraldehyde-3-phosphate dehydrogenase at pH 6.1 ?[ The isoelectric point of glutamic acid is 3.22; asparagine, 5.41. During paper electrophoresis at pH 6, toward which electrode does glutamic acid migrate? | During paper electrophoresis at pH 6.5, toward which electrode does asparagine migrate? [
The catalytic activity of an enzyme is being measured at pH 7.5 and requires that the side chain of a specific Cys (C) residue be in its unprotonated (conjugate base) form for the enzyme to be active. That specific Cys side chain in the protein has a pKa of 7.8. a) For this Cys side chain with a pKa of 7.8, what is the base/acid ratio at pH 7.5? b) What fraction of the total enzyme molecules in a solution of the enzyme at pH 7.5 have that Cys side chain in the active form? (Express the answer as a %) c) The name of the Cys side chain functional group is? d) The active form of the Cys side chain in this enzyme would be? i. positively (+) charged ii. uncharged (neutral) iii. negatively (–) charged
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