The concentration of acetylsalicylic acid in an antipyretic tablet was determined by HPLC using a normal calibration curve. Standard solutions of acetylsalicylic acid were prepared and analysed using a 10uL loop. The results obtained for the standards are tabulated below: Conc. of Acetylsalicylic Acid (ppm); Signal (arbitrary units) 50; 6352 100; 12869 150; 19175 200; 25536 250; 31936 To determine the concentration of acetylsalicylic acid in an antipyretic tablet, 10 tablets were weighed (6.500 g total) and crushed to a fine powder using a mortar and pestle. A quantity of powder equal the mass of one tablet was added to a 100 mL volumetric flask. The tablet powder was dissolved with 20 mL of methanol and the flask was made to volume using water. The solution was filtered. A 1.000 ml aliquot was transferred to a 10 mL volumetric flask, which was then made to volume with water. Analysis was conducted using a Bio Sil HL C18, 250mm x 4.6mm column; 5um, with a mobile phase of acetonitrile: water (25:75) at 2.0 mL/min. UV detection was at 207 nm. When analysed by HPLC, the signal for acetylsalicylic acid was found to be 20895. Report the amount of acetylsalicylic acid (in mg) in one antipyretic tablet. Was the analysis conducted using normal or reverse phase chromatography?
The concentration of acetylsalicylic acid in an antipyretic tablet was determined by HPLC using a normal calibration curve. Standard solutions of acetylsalicylic acid were prepared and analysed using a 10uL loop. The results obtained for the standards are tabulated below: Conc. of Acetylsalicylic Acid (ppm); Signal (arbitrary units) 50; 6352 100; 12869 150; 19175 200; 25536 250; 31936 To determine the concentration of acetylsalicylic acid in an antipyretic tablet, 10 tablets were weighed (6.500 g total) and crushed to a fine powder using a mortar and pestle. A quantity of powder equal the mass of one tablet was added to a 100 mL volumetric flask. The tablet powder was dissolved with 20 mL of methanol and the flask was made to volume using water. The solution was filtered. A 1.000 ml aliquot was transferred to a 10 mL volumetric flask, which was then made to volume with water. Analysis was conducted using a Bio Sil HL C18, 250mm x 4.6mm column; 5um, with a mobile phase of acetonitrile: water (25:75) at 2.0 mL/min. UV detection was at 207 nm. When analysed by HPLC, the signal for acetylsalicylic acid was found to be 20895. Report the amount of acetylsalicylic acid (in mg) in one antipyretic tablet. Was the analysis conducted using normal or reverse phase chromatography?
Chapter14: Chromatography
Section: Chapter Questions
Problem 9P
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Transcribed Image Text:The concentration of acetylsalicylic acid in an antipyretic tablet was determined by HPLC using a normal calibration curve. Standard solutions of acetylsalicylic acid were prepared and analysed using a 10µL loop. The results obtained
for the standards are tabulated below:
Conc. of Acetylsalicylic Acid (ppm); Signal (arbitrary units)
50; 6352
100; 12869
150; 19175
200; 25536
250; 31936
To determine the concentration of acetylsalicylic acid in an antipyretic tablet, 10 tablets were weighed (6.500 g total) and crushed to a fine powder using a mortar and pestle. A quantity of powder equal the mass of one tablet was
added to a 100 mL volumetric flask. The tablet powder was dissolved with 20 mL of methanol and the flask was made to volume using water. The solution was filtered. A 1.000 ml aliquot was transferred to a 10 ml volumetric flask,
which was then made to volume with water.
Analysis was conducted using a Bio Sil HL C18, 250mm x 4.6mm column; 5um, with a mobile phase of acetonitrile: water (25:75) at 2.0 mL/min. UV detection was at 207 nm.
When analysed by HPLC, the signal for acetylsalicylic acid was found to be 20895. Report the amount of acetylsalicylic acid (in mg) in one antipyretic tablet. Was the analysis conducted using normal or reverse phase chromatography?
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