Select all the statements that provide evidence that DNA is the genetic material. Check All That Apply By weight, a eukaryotic chromosome contains more protein than DNA. Treatment of purified DNA with a DNA-degrading enzyme destroyed the ability of S bacteria to transform R bacteria. The R mutant form of S. pneumoniae does not cause infection in mice. Radioactively-labeled DNA remained in bacterial cells after phage T2 infection. Proteins are more complex than DNA because they are composed of 20 different amino acids.

Select all the statements that provide evidence that DNA is the genetic material. Check All That Apply By weight, a eukaryotic chromosome contains more protein than DNA. Treatment of purified DNA with a DNA-degrading enzyme destroyed the ability of S bacteria to transform R bacteria. The R mutant form of S. pneumoniae does not cause infection in mice. Radioactively-labeled DNA remained in bacterial cells after phage T2 infection. Proteins are more complex than DNA because they are composed of 20 different amino acids.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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Many microbes (as well as other organisms) produce antibiotics. A natural compound produced by one organism that inhibits or kills another unrelated organism is commonly defined as an antibiotic. Thousands of antibiotics have been identified and characterized. Antibiotics prevent virtually every step in the Central Dogma of bacteria. Rifampicin, for example, inhibits transcription while streptomycin inhibits translation. However, no antibiotic has ever been discovered that directly affects DNA replication by inhibiting the action of DNA polymerases. "Why not?"
Select all the statements that provide evidence that DNA is the genetic material. Check All That Apply 1. By weight, a eukaryotic chromosome contains more protein than DNA. 2. Treatment of purified DNA with a DNA-degrading enzyme destroyed the ability of S bacteria to transform R bacteria. 3. The R mutant form of S. pneumonia does not cause infection in mice. 4. Radioactively-labeled DNA remained in bacterial cells after phage T2 infection. 5. Proteins are more complex than DNA because they are composed of 20 different amino acids. Pls help asap!! I am so confused
SSBs are: single-stranded DNA binding proteins that prevent re-annealing supercoil stabilizing bodies. single-stranded bodies called Okazaki fragments. substrates for DNA ligases.
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
In your own words, explain how bacteria can be manipulated to contain a foreign DNA sequence or gene, how the expression of the gene could be monitored or selected for in the lab, and how the protein coded from this DNA sequence could potentially be purified. You must include at least 6 of the following terms in your answer: transformation, plasmid, antibiotic selection, fusion protein, chromatography, reporter gene, bioluminescence, competent cells, electroporation, calcium chloride.
Researchers are designing several experiments to test the ability of Salmonella bacteria to develop antibiotic resistance. A culture of Salmonella bacteria is exposed to the same concentrations (200 mg/L) of an antibiotic for four days. The table shows the number of isolated resistant bacteria over a four-day period. Which of the following statements best explains these results? A - The bacteria were not affected by the antibiotic. B - After being exposed to the antibiotic, the bacteria altered their DNA. C - A new species of bacteria emerged after the antibiotics were introduced. D - Random mutations led some bacteria to be resistant and, over time, they increased in the population.
The result Jake obtained after transforming his competent Escherichia coli cells with plasmids is as shown below: Is the transformation experiment conducted a success? Justify your answer.
Which of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true? Only NER involves the action of DNA ligase to seal nicks in the DNA backbone. Both NER and BER involve DNA strand cleavage by an endonuclease. Both NER and BER can be activated by exposure to visible light. Only BER requires DNA polymerase. Both NER and BER involve the creation of an apyrimidinic (AP) site.
Define the following terms: Genome Bacteriophage λ DNA Plasmid Restriction Enzyme Standard Curve Plasmid Map
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).
In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select one: confirm which plasmids been have successfully ligated with a gene of interest. isolate bacteria which have been successfully transformed with the plasmid. indicate which plasmids were successfully digested by the endonuclease. act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful. show which plasmids contain the lacZ gene.