Select all that would be true if I had a missense mutation in an gene: The missense mutant protein would be the same size by Western as the wildtype protein The missense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The missense mutant protein would be a different size by Western compared to the wildtype protein The missense mutant allele would be the same size as wildtype by PCR-electrophoresis

Select all that would be true if I had a missense mutation in an gene: The missense mutant protein would be the same size by Western as the wildtype protein The missense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The missense mutant protein would be a different size by Western compared to the wildtype protein The missense mutant allele would be the same size as wildtype by PCR-electrophoresis

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications

Section: Chapter Questions

Problem 1ITD: You learned in the chapter that an STR locus is a locus where alleles differ in the number of copies...

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Select all that would be true if I had a nonsense mutation in an exon of a gene: The nonsense mutant allele would be the same size as wildtype by PCR-electrophoresis The nonsense mutant protein would be the same size by Western as the wildtype protein The nonsense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The nonsense mutant protein would be a different size by Western compared to the wildtype protein
The results of the diagram (Agarose electrophoresis photodocumentation and quantification table) correspond to the information for 4 samples of genomic DNA extracted from plant tissue. With which sample (s) could a PCR be performed to amplify a 100 pb region? Al A2 АЗ А4 ID DNA 260 nm 260 nm (pb) 10,000 sample (ng/uL) 280 m 230 nm A1 170 1.9 2.0 А2 180 1.5 1.5 5,000 АЗ 170 1.7 1.5 А4 170 1.9 2.0 1,000 500 100 choose the best option: а. All b. None с. А2 d. A1, A2 е. А1
For separating DNA of different sizes, you would use Question PCR Questi Gel electrophoresis Restriction enzymes 1 Listen Crispr For making specific DNA changes in living organisms, you would use ** Crispr Restriction enzymes Gel electrophoresis PCR ◄0 Listen You are interested in identifying genes that determine tail length in dachsunds. You analyze the genome of several dachsunds and look for SNPS that correlate with tail length. The results are shown here pog Spot Rover Chase Frisbee Cin Checkers SNP 1 SNP 2 SNP 3 Tail Length (cm) 10 12 SNP 4 9 4 3 10 SNP 1 C C C G C SNP 2 A T T A A T Based on these results which SNP is best correlated with tail length SNP 3 C C C G C SNP 4 G G T T T G
This is what the LAB FLOW would look like: CULTURE BACTERIA EXTRACT RNA RANDOM HEXAMER REVERSE TRANSCRIPTION OF RNA PCR OF lacZ GENE ELECTROPHORESIS If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube? What would be the final primer concentration if 0.5 μl of 10 μM primers were added to a PCR reaction with a final volume of 20 μl?
For the STR site diagramed below, each repeat unit, represented by a rectangle, is known to be 5 bp long. The total length of the amplified PCR product for the chromosome shown below is 420 bp long. PCR product - 420 bo Primer Left STR STR STR STR Primer Right What size would the PCR product be for a different chromosome where there were only two tandem repeat units, instead of four? SHOW YOUR WORK.
The temperature at which the primers and target DNA hybridize may be changed to influence the stringency of PCR amplification. What effect will changing the hybridization temperature have on the amplification? Let's say you have a certain yeast gene A and want to check whether it has a human equivalent. How might managing the hybridization's rigor benefit you?
What would be the expected effect on the PCR reaction, if you increased the temperature of the annealing phase and the length of the elongation phase? O Accuracy will be reduced, but yield will be increased Accuracy and yield will be increased OAccuracy will be increased, but yield will be decreased Accuracy and yield will be reduced
Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices Errors could have been incorporated during restriction enzyme digestion and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during plasmid DNA extraction and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during PCR and so it is important to verify that only the expected protein is produced. Errors could have been incorporated during cloning and so it is important to verify that only the expected protein is produced.
Make the PCR Cocktail I field out my work.. but I don't know which one is incoeert.. This table lists the ingredients, stock reagent concentrations, and concentrations in the PCR reaction. Prepare a "PCR cocktail" to be added to your samples to achieve these concentrations. Make enough cocktail to run nine samples. [Four student samples + three positive controls + one negative control + one extra.] {Hint: Remember that the concentration in the reaction is not the same as the concentration in the cocktail!] Component Stock Concentration Concentration in the PCR reaction Volume per reaction Volume to make cocktail Sterile water - - 0µl 0µl PCR buffer w/ MgCl2 10x 1x 4µl 36µl Nucleotide mix 10 mM 0.2 mM 0.8µl 7.2µl Primer 1 (Forward) 10 µM 1.0 µM 4µl 36µl Primer 2 (Reverse) 10 µM 1.0 µM 4µl 36µl Taq DNA polymerase 5 U/µl 1.0 U 8µl 72µl DNA template (sample) - ~1 ng 20 µl 180µl Total - - 40 µl 360 µl
Regarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genome
Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples didn’t already contain dye and you wanted to load your PCR sample onto an agarose gel, you’d need to add loading dye to the proper concentration. There is a 6X loading dye available for use; how many µl of this loading dye will you add to 10 µl of your sample so that it is at a 1X working concentration? Show your work.
Below are several problems frequently faced by researchers when running the PCR. Give one (1) solution to each problem stated. 1. No PCR product 2. Multiple bands appeared after gel electrophoresis (you are amplifying just 1 gene) 3. Bright thick bands at the end of the agarose gel after electrophoresis 4. Bands appeared at the negative control