Please provide a detailed solution for the following question You have constructed a standard curve from BSA protein for a Bradford Assay. The curve has 0, 1, 2, 3, and 4 ug protein in 50 µl water. You have an unknown protein with a concentration somewhere between 1-5 mg/mL. What serial dilution series should you use to guarantee at least two dilutions of your unknown fall below 3 ug (linear portion) on the standard curve? For example, would 1/2 x 1/2 x 1/2 (3 2-fold dilutions) be sufficiently dilute that two of the dilutions would give values within the linear portion of the standard curve?

Please provide a detailed solution for the following question You have constructed a standard curve from BSA protein for a Bradford Assay. The curve has 0, 1, 2, 3, and 4 ug protein in 50 µl water. You have an unknown protein with a concentration somewhere between 1-5 mg/mL. What serial dilution series should you use to guarantee at least two dilutions of your unknown fall below 3 ug (linear portion) on the standard curve? For example, would 1/2 x 1/2 x 1/2 (3 2-fold dilutions) be sufficiently dilute that two of the dilutions would give values within the linear portion of the standard curve?

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter16: Applications Of Neutralization Titrations

Section: Chapter Questions

Problem 16.18QAP

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