ns) You use paper chromatography to separate the following two molecules. The stationary phase is coated with hexane CH14 and the mobile phase is water. Which molecule will travel furthest up the paper? Explain using intermolecular forces between molecule and water. OH Phenol Napthol
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- In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?Separate a mixture of Vitamin D and Vitamin B2 (riboflavin) by paper chromatography. The stationary phase is methanol (CH;OH) and the mobile phase is pentane (C;H12). Place the vitamins in order of elution. Explain why using IMF. (Circle polar sites on each molecule and label all 4 molecules as polar or non-polar.) Vitamin Ba 0: H3C NH H3C CH3 CH3 CH3 H3C He H3C HO CH2 Vitamin D He CH2OH HO
- ef Correlate the structures of the standard food dyes to their relative position in the paper chromatogram. Refer to the structures shown below. H3C. *Na O O HO. O CH3 Allura Red AC (pigment in red dye) 01510 106 FO O Nat O Nat O Nat FO O Na+ 0=8=0 HO 0=S=0 Na Na+ Tartrazine (pigment in yellow dye) Brilliant Blue FCF (pigment in blue dye) What are the identities of the unknowns in paper chromatography of food dyes? How did you arrive at this conclusion? Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the experiment you did in thin layer chromatography of plant pigments? How do you say so?Please propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.A student performed a chromatography experiment to determine the Rf value of an unknown amino acid. The distances traveled by the amino acid and the solvent line were 3.0 cm and 8.5 cm, respectively. What is the Rf value for this amino acid? 0.3 cm O 0.35 1.8 5.5 0.85 cm 0.65 O 2.8
- Q2) In the thin layer chromatography experiment, calculate the relative mobility for the two dyes if the distance traveled by the solvent (ethanol) was 3 cm, the distance traveled by the purple dye was 2.5 cm, and 1.5 cm for the red dye? Which dye is considered less soluble in ethanol?What would the gc chromatogram look like when 6.65 g NaBr, 3.78 g NaCl, 7.5 mL of DI water and 5 mL of 1‐butanol are added together then 6 mL of concentrated sulfuric acid added.Question 32 Use the following chromatogram of standard amino acids: 24 11 16 19 3 12 21 15 6, 8 9 19 23 2 13 14 17 18 24 22 10 12 14 16 18 20 22 24 26 Time (min) Which of the following statements BEST describe the given chromatogram? O Peaks 4, 5, 11 and 24 have the highest concentrations in the mixture and highest affinity to the mobile phase. Peaks 14, 18, 20 and 22 have the lowest concentrations and highest affinity to the mobile phase. O The peaks are narrow and show clear separation of the mixture. The peaks are narrow and show suboptimal conditions for separation. (Au)
- use the following chromatogram and table as your data. What is the %composition of the Vitamin A peak in the sample? 78.43% 77.73% 7.867% 72.74%How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solvent2. Consider the following example GC Chromatogram from a publication about aggression among Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of unique compunds present in the sample? What data led you to that conclusion? (Don't overthink it) 2 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 Counts (Abundance) 5 19 6 7 0 23.00 24.00 10 | 11 13 17 20 14 15 16 12 teel | 22 19 21 ли 25.00 26.00 27.00 Time "Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo Frizzi, et.al, in PLoS One is licensed under CC BY 4.0