Kinetic assays were performed to better understand the activity of the enzyme 245C in the absense and presence of molecule B. The enzyme follows Michaelis-Menten kinetics. a) Calculate the VO for this enzyme when [S]=0.03mM and no molecule B is present. b) Using a properly labelled Lineweaver-Burke plot with accurate x- and y-intercepts, describe the effect of molecule B on the enzyme. Briefly explain how molecule B could be causing these effects

Kinetic assays were performed to better understand the activity of the enzyme 245C in the absense and presence of molecule B. The enzyme follows Michaelis-Menten kinetics. a) Calculate the VO for this enzyme when [S]=0.03mM and no molecule B is present. b) Using a properly labelled Lineweaver-Burke plot with accurate x- and y-intercepts, describe the effect of molecule B on the enzyme. Briefly explain how molecule B could be causing these effects

Chemistry: Principles and Practice

3rd Edition

ISBN:9780534420123

Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer

Chapter1: Introduction To Chemistry

Section: Chapter Questions

Problem 1.102QE

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Mn→ Mn2+ + 2e Mn2+ Mn³+ + e Mn3+ + 2H₂O → MnO2 + 4H+ + e MnO2 + 2H₂O → MnO42- + 4H+ + 2e- MnO42- MnO4 + e Mn2+ + 2H₂O → MnO2 + 4H+ 2e Eº = -1.23 V MnO2 + 2H2O → MnO4 + 4H+ + 3e Eº = -1.70 V Mn2+ + 4H20 MnO4 + 8H+ + 5e EO = -1.51 V - i. Summarise the above potential data by means of a Latimer and Frost diage EO = +1.18 V Eº = -1.51 V EO = -0.95 V EO = -2.26 V Eº = -0.56 V
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Would this be solved using n=MV??