he DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. A. Human DNA is approximately 20% C. G = % A = % T = % B. In the bacteriophage ΦΧ174, the DNA is approximately 26% A. C = % G = % T = % C. Sea urchin DNA is approximately 18% G. C = % A = % T = %
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2. The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing.
A. Human DNA is approximately 20% C.
G = % A = % T = %
B. In the bacteriophage ΦΧ174, the DNA is approximately 26% A.
C = % G = % T = %
C. Sea urchin DNA is approximately 18% G.
C = % A = % T = %
Step by step
Solved in 3 steps
- 2. A. B. C. G=% A = % T= % 6 In the bacteriophage DX174, the DNA is approximately 26% A. C= % G= % T = % Sea urchin DNA is approximately 18% G. C = % A = % T = % 6 17 19 10 11 The DNA of each species has a different base composition. Find the base composition of each species, using what you know about complementary base pairing. Human DNA is approximately 20% C. 12 13 Go Ent Into3. The data in the following table represent the base composition of two double stranded DNA sources and their RNA products in experiments conducted in vitro. A+T A+U A+G Species G+C G+C C+U Bacillus subtilis 1.36 |1.30 1.02 E. coli 1.00 |0.98 0.80 From the above data, is it possible to determine whether the RNA of these species is copied from a single strand or from both strands of the DNA? How? (HINT: Drawing a diagram may make it easier to solve this problem). Explain how it is possible to tell whether the RNA itselfis single stranded or double stranded.6. Which of the following DNA sample composition is expected to have the highest boiling point?A. 60% GC pair and 40% AT pairB. 50% GC pair and 50% AT pairC. 80% GC pair and 20% AU pairD. 20% GC pair and 80% AU pair7. What is the sequence following compounds in terms of increasing solubility to dilute methanol solution?A. Adenine → Adenosine → Adenosine monophosphateB. Adenosine monophosphate → Adenosine → AdenineC. Adenine →Adenosine monophosphate → AdenosineD. Adenosine diphosphate → adenine → Adenosine triphosphate
- 6. Which of the following DNA sample composition is expected to have the highest boiling point?A. 60% GC pair and 40% AT pairB. 50% GC pair and 50% AT pairC. 80% GC pair and 20% AU pairD. 20% GC pair and 80% AU pair7. What is the sequence following compounds in terms of increasing solubility to dilute methanol solution?A. Adenine → Adenosine → Adenosine monophosphateB. Adenosine monophosphate → Adenosine → AdenineC. Adenine →Adenosine monophosphate → AdenosineD. Adenosine diphosphate → adenine → Adenosine triphosphate8. Which RNA molecule is NOT involved in the processes of transcription and translation?A. Messenger RNAB. Transfer RNAC. Ribosomal RNAD. Micro-RNA 9. Which of the following functional groups promote the separation of nucleotide fragments in gel electrophoresis?A. Phosphate groupsB. Ribose moietiesC. Purine basesD. Pyrimidine bases10. How many nucleosomes are there in 6 solenoids?A. 10B. 42C. 36D. 501. Suicide enzyme will take in the alkyl group through its carbon atom from a cysteine residue. a. True b. False Answer: 2. These are the consequences of the changes in the messenger codon nucleotide sequence. a. Recombination b. Mutation c. Disease d. Abnormality Answer: 3. A.) Damaged DNA can be reversed if nucleotides can be replaced with a proper nucleotide for a correct amino acid base. B.) Direct reversal of DNA repair can destroy the abnormal bonds of the nucleotides in the DNA sequence. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrect Answer:1. Written below is a DNA seqeunce: G G C A A C T A T C C C G A T T A G C G C Write down the sequence for the complimentary DNA sequence 2. Written below is the DNA sequence of a gene: T A C C T A A G C G C C G G T C A T A T C A. Write down the mRNA sequence that would be transcribed from this DNA B. Write down the amino acid sequence that would be translated from the mRNA sequence 3. Written below is the DNA sequence of a gene: T A C G T G T T T A C T C C A C A T G A T A T C A. Write down the mRNA sequence that would be transcribed from this DNA B. Write down the amino acid sequence that would be translated from the mRNA sequence
- 2.Fill in the blank with the best answer. If band 3 (6 kB) in lane 5 contains 280 ng of DNA, then band 6 (2.1 kB) must contain mg of DNA. (use scientific notation). Ethidium bromide (use the most scientific term) between the in the DNA; a band that is 60 kB will be than a 40,000 bp band in the same lane; if the 60 kb band contains 0.02 ug of DNA per intense kB, than the band that is 40,000 bp in the same lane will contain ng of DNA, or ng/kB. The chemical structure of ethidium bromide has that make it look like the in DNA.5. The nucleotide sequences of the DNA molecules in the figure below were obtained from four different individuals, one wild type and three mutants. Wild Type 5'-TTATCCATGATCGGATCGATCCATTAGCCGA-3' 3'-AATAGGTACTAGCCTAGCTAGGTAATCGGCT-5’ Mutant I 5'-ATCCATGATCGGATTGATCCATTAGCCGAAT-3’ 3'-TAGGTACTAGCCTAACTAGGTAATCGGCTTA-5’ Mutant II 5'-CCGTTATCCATGATCGGATAGATCCATTAGCC-3’ 3'-GGCAATAGGTACTAGCCTATCTAGGTAATCGG-5’ Mutant III 5'-CACCGTTATCCATGATCGGAACGATCCATTAGC-3’ 3'-CAGGCAATAGGTACTAGCCTTGCTAGGTAATCG-5’ a) Identify the open reading frames in each sequence of DNA and translate them into proteins. Write down the sequence of amino acids that will be obtained after translation: b) Which of the mutations above would be least likely to cause a change in the function of the protein? Why? c) Which of the mutations above would probably cause a major disruption in the function of the protein? Why?1. Which of the following are generated due to the twisting of dsDNA? a. Hydrophobic bonds b. Van der Waal forces c. Hydrogen bonds d. Grooves 2. Which of the following terms refers to the annealing of ssDNA to complementary RNA? a. Homologous combination b. Hybridization c. Denaturation d. Telomere lengthening 3. Which of the following completes this statement? The melting temperature of DNA is the temperature at which__________. a. DNA denatures b. DNA bonds are reinforced c. Solid DNA becomes liquid d. Liquid DNA coils
- 1. On paper, write out the first 30 bases in the middle of the page in order. You want ensure you get them all in one line, with room to write more information around them. 2. Fill in the complementary base for each of the 30 bases. Label this series of letters “Complementary Bases” on the paper. When you are done, you should have a complete double-helix section of DNA.3. On the opposite side of the original sequence (opposite from where you did the complementary strand), translate the original sequence into mRNA. Label this sequence as mRNA.4. Translate the mRNA strand into amino acids using your codon table. You only need to record the 3-letter code for each amino acid. Label this line as “Amino Acids.” Record this sequence in your lab report (question 8).5. Take a picture of your completed diagram for your lab report. Part 2 – Mutations You will use the same 30-base sequence you started with for this section. 1. Write out the original 30-base DNA sequence on a new paper.2.…2. Tube A contains 800 µl of a DNA solution that has a concentration of 345 µg/ml. a. You transfer 0.2 ml of DNA solution from Tube A and place it into Tube B. How much total DNA is present in Tube B?1. During the replication of a DNA molecule, separation or "unzipping" of the DNA molecule will normally occur when hydrogen bonds are broken between a. thymine and thymine b. guanine and uracil c. adenine and cytosine d. cytosine and guanine 2. The individuality of an organism is determined by the a. sequence of nitrogenous bases in deoxyribonucleic acid b. number of amino acids in a cell c. position of the ribosomes on the endoplasmic reticulum d. number of nitrogenous bases in a codon 3. The weakest bonds in a double-stranded molecule of deoxyribonucleic acid exist between the a. deoxyribose sugars b. phosphate groups C. nitrogenous bases d. 5-carbon sugars
