Explain why TSIA is a suitable medium for identifying enteric bacteria. To do this you will have to find out how enteric bacteria react with the ferrous sulfate in the TSIA.
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Explain why TSIA is a suitable medium for identifying enteric bacteria. To do this you will have to find out how enteric bacteria react with the ferrous sulfate in the TSIA.
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- A stool specimen of a patient with severe diarrhea was cultured in a series of specialized media for isolation of enteric organisms. The cultures yielded three isolates that were species of Salmonella, Shigella, and Escherichia. Explain why the Hydrogen sulfide production test would be diagnostically significant.EXPLAIN THE PURPOSE OF THE METHYL RED AND VOGES-PROSKAUER TESTS IN DETERMINING THE IDENTITY OF GRAM NEGATIVE ORGANISMS IN THE FAMILY ENTEROBACTERIACEAE. INCLUDE IN YOUR ANSWER WHY METHYL RED IS USED RATHER THAN PHENOL RED. WHAT ORGANISM IN THE FAMILY IS USED AS A CONTROL WHEN RUNNING THE METHYL TEST. IF THE METHYL RED TEST IS NEGATIVE, EXPLAIN WHY THE VOGES-PROSKAUER TEST IS RUN AND INCLUDE WHAT COLOR CHANGE INDICATES A POSITIVE TEST. ACCOUNT FOR THE COLOR DIFFERENCES IN THE NEGATIVE VOGES-PROSKAURER TESTS. WHAT REAGENTS (COMMON NAME) WERE USED IN THE VOGES -PROSKAUER TEST.EMB agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. It is particularly effective at determining if E. coli is present in a specimen. Define what type(s) of media EMB agar is, using as many terms in the answer list as are applicable.
- Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarYour organism is a Gram-negative organism (Enterobacter aerogenes) You must discuss all the IMViC tests and Hydrogen sulfide test in DETAIL. How can this be explained in lamen terms the step by step results of enterobacter aerogenes? DISCUSS all the results. Discuss in a logical and step by step fashion how you concluded that you had a certain microorganism.You need to explain which observation(s) enabled you to identify your unknown microorganism (Enterobacter aerogenes).To convince your reader you need phrases like; This observation “suggested” that my organism is Enterobacter aerogenes This observation “confirmed” that my organism is Enterobacter aerogenesMcConkey agar red is considered to be both differential and selective for the isolation of members of the family Enterobacteriaceae. Explain why
- You must write up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain and the second will be Gram negative. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes. Can you please help me find the dichotomous key . Thank you !If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol.Vibrio cholerae can be cultured on Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS). Enterobacteriaceae and gram-positive bacteria are inhibited on this agar. cholerae produces large yellow colonies while other Vibrio species produce blue, green, or blue-green colonies. Discuss the purpose of each media listed. Based on this information alone, what term(s) would best describe this agar? Selective Enrichment (enriched) Differential Reducing Selective and enriched Selective and differential Selective, differential, and enriched
- A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?A stool specimen of a patient with severe diarrhea was cultured ina series of specialized media for isolation of enteric organisms. Thecultures yielded three isolates that were species of Salmonella, Shigella,and Escherichia. Explain why the H2S production test would be diagnostically significant.Why does a precipitate form on Sierra’s medium when inoculated with a lipolytic organism? Discuss the principle behind this procedure.