Examine the given table below. What interpretation can be drawn from the data regarding the test disinfectant? Explain and Justify your points Disinfectant Phenol Dilution 1:95 1:100 1:105 1:110 1:115 Growth after 5 minutes Growth after 10 minutes Growth after 15 minutes Disinfectant Test disinfectant Dilution 1:100 1:125 1:150 1:175 1:200 Growth after 5 minutes . + . Growth after 10 minutes . + Growth after 15 minutes . +

Examine the given table below. What interpretation can be drawn from the data regarding the test disinfectant? Explain and Justify your points Disinfectant Phenol Dilution 1:95 1:100 1:105 1:110 1:115 Growth after 5 minutes Growth after 10 minutes Growth after 15 minutes Disinfectant Test disinfectant Dilution 1:100 1:125 1:150 1:175 1:200 Growth after 5 minutes . + . Growth after 10 minutes . + Growth after 15 minutes . +

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 8.1P

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ANSWER THE FOLLOWING QUESTIONS REGARDING GEL ELECTROPHORESIS 1.why is it not advisable to move/touch the agarose gel in the process of hardening 2.what is the use or function of the TAE buffer that is poured or found in the gel box 3.how can you tell if agel is running 4.outline the process use in the preparation of agarose gel of 1.5 concentration 5.outline the processes/steps used in gel electrophoresis 6.name twoexamples of the dye used in gel electrophoresis 7.how do we prepare x1(concentration) TAE BUFFER form 50x TAE buffer
8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?
Determine the CFU/ml of the original culture in the image below. Write your value in scientific notation using the "^" symbol to denote an exponent. (Example: 1x10^3). Write the number only (no labels, like CFU/ml). 100 μ. . A Original Culture 1.2x10^6 100 μ. 100 μ. Lawn of growth 100 μ. B΄900 ul c|900 μl | D |900 μ. € 900 μ. | 100 με 1100 με 1100 με 1100 μ. 1,198 120 colonies colonies O 00 10 colonies
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As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the number of colony forming units (CFU) per mL it contains. You do a plate count with these growth results (no. of colonies for each dilution): 1:10 too many to count; 1:100 too many to count; 1:1,000 174; 1:10,000 23; 1:100,000 no growth. The number of CFU per mL in the original culture was: 1 ml 1 ml Original inoculum Dilutions 9 ml broth in each tube 1:10 1 ml 174,000 1:100 1 ml 1 ml 1 ml 1:1000 1 ml 1:10,000 1 ml None of the other four answers (Correct answer not given) 1,000 230,000 174 1 ml 1:100,000 1 ml
The total number of cells in a culture is counted using the trypan blue exclusion assay and is found to be 6.8 x 106 cells/ml. Each well in a 6 well plate requires 2 x 105 cells. How should the solution be diluted so that 1ml can be added to each well?
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Calculate the estimated concentration of test agent that inhibit cell growth by 50% ( GI50) value
A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 1. diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies