Enhancer sequences may be very far from the genes they affect but they are always upstream of the gene sequence. True False
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- Noncoding ribonucleic acid (RNA) interference/regulation in reguards to gene expression. Provide examples.The pre-mRNA transcript and protein made by several mutant genes were examined. The results are given below. Determine where in the gene a likely mutation lies: the promoter region, exon, intron, cap on mRNA, or ribosome binding site. a. normal-length transcript, normal-length nonfunctional protein b. normal-length transcript, no protein made c. normal-length transcript, normal-length mRNA, short nonfunctional protein d. normal-length transcript, longer mRNA, shorter nonfunctional protein e. transcript never made1. You are investigating a protein that has the amino acid sequence N ... Ala – Thr - Asn – Trp – Lys - Arg - Gly – Phe – Thr ... C within its primary structure. You found that several of the mutations affecting this protein produced shortened protein molecules that terminated within this region. In one of the mutants, the Asn became the terminal (last) amino acid. (a) What DNA single-base changes(s) would cause the protein to terminate at the Asn residue? (b) What other potential sites do you see in the DNA sequence encoding this protein where mutation of a single base pair would cause premature termination of translation? >
- A protein has the following amino acid sequence: Met-Tyr-Asn-Val-Arg-Val-Tyr-Lys-Ala-Lys-Trp-Leu-Ile-His-Thr-Pro You wish to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Your probes should be at least 18 nucleotides in length. Q. Which amino acids in the protein should be used to construct the probes so that the least degeneracy results?Below is the double stranded DNA sequence of part of a hypothetical yeast genome encoding a very small gene. Transcription starts at nucleotide immediately following the promoter. The termination sequence is TATCTC. How many amino acids will this protein have? 5' TCATGAGATA GCCATGCACTA AGGCATCTGA GTTTATATCT CA 3' 3' AGTACTCTAT CGGTACGTGAT TCCGTAGACT CAAATATAGA GT 5'Please help, doesn't it usually start before the promoter or after, please just give me a brief explanation
- You are working for a pharmaceutical company are are tasked with creating E. Coli that express an influenza antigen (a protein that is recognized by our immune system) fortreatment of the influenza virus. The cDNA of the antigen is available in a carrier vector(pCARRY) that contains a kanamycin resistance gene as shown on the left below. Inorder to get E. Coli to express the antigen, you need to clone the gene for the influenzaantigen into an expression vector that contains an E. Coli-specific promoter. You obtain such an expression vector, which contains an ampicillin resistance gene as shown on the right below (pBACTERIA) a. An end generated by digestion with BamH1 can be ligated to an end generated by digestion with BlgII. Why is this possible? b. To clone the gene for the influenza antigen from pCARRY into pBACTERIA plasmid:i. What restriction enzyme should you use to digest pCARRY? ii. What restriction enzyme should you use to digest pBACTERIA? c. After you ligate the products of…A protein has the following amino acid sequence: Met-Tyr-Asn-Val-Arg-Val-Tyr-Lys-Ala-Lys-Trp-Leu-Ile-His-Thr-Pro You wish to make a set of probes to screen a cDNA library for the sequence that encodes this protein. Your probes should be at least 18 nucleotides in length. Q. How many different probes must be synthesized to be certain that you will find the cDNA sequence that specifies the protein?GCT GAC ATC CTC CTC mutated DNA sequence mutated mRNA sequence mutated amino acid sequence Above is the mutated DNA sequence for part of the 21 hydroxykase gene. Provide the mutated mRNA and amino acid sequence.
- Based on the electron micrograph shown, which of the following statements is/are correct/true? Select all that apply. You may select mutiple options. DNA and mRNAs are marked by arrows. DNA mRNAs #3 Left Right 1 μm You cannot determine relative mRNA lengths from this image You cannot determine the direction of transcription from this image the longest mRNA is on the right side ✔Direction of transcription is left to right O Direction of transcription is right to left the 5' end of the mRNA marked #3 is at the bottom O the longest mRNA is on the left side the 5' end of the mRNA marked #3 is at the top You cannot determine the 5' or 3' mRNA ends from this imageTRUE OR FALSEErrors in transcription can lead to silent mutations encoding the same amino acid or an altered protein that retains its functionality.True/False: In general, weak promoters that support active transcription have -10 and -35 boxes that are close to the consensus sequences and a spacer between the two boxes that is 17 +/- 1 bp.