Electron transfer translocates protons from the mitochondrial matrix to the external medium, establishing a pH gradient across the inner membrane (outside more acidic than inside). The tendency of protons to diffuse back into the matrix is the driving force for ATP synthesis by ATP synthase. During oxidative phosphorylation by a suspension of mitochondria in a medium of pH 7.4,pH 7.4, the measured pH of the matrix is 7.7. Calculate [H+] in the external medium and in the matrix under these conditions. a)[H+]in the matrix: b)What is the outside‑to‑inside ratio of [H+]? c)What is the free‑energy change inherent in this concentration difference across the membrane? (Assume a temperature of 25 °C.25 °C.)
Electron transfer translocates protons from the mitochondrial matrix to the external medium, establishing a pH gradient across the inner membrane (outside more acidic than inside). The tendency of protons to diffuse back into the matrix is the driving force for ATP synthesis by ATP synthase. During oxidative phosphorylation by a suspension of mitochondria in a medium of pH 7.4,pH 7.4, the measured pH of the matrix is 7.7. Calculate [H+] in the external medium and in the matrix under these conditions. a)[H+]in the matrix: b)What is the outside‑to‑inside ratio of [H+]? c)What is the free‑energy change inherent in this concentration difference across the membrane? (Assume a temperature of 25 °C.25 °C.)
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Electron transfer translocates protons from the mitochondrial matrix to the external medium, establishing a pH gradient across the inner membrane (outside more acidic than inside). The tendency of protons to diffuse back into the matrix is the driving force for ATP synthesis by ATP synthase. During oxidative phosphorylation by a suspension of mitochondria in a medium of pH 7.4,pH 7.4, the measured pH of the matrix is 7.7.
Calculate [H+] in the external medium and in the matrix under these conditions.
a)[H+]in the matrix:
b)What is the outside‑to‑inside ratio of [H+]?
c)What is the free‑energy change inherent in this concentration difference across the membrane? (Assume a temperature of 25 °C.25 °C.)
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