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- You first separated the components of spinach and then identified them by TLC analysis. Was there a correlation between the order in which the components were collected and the distance they traveled on the TLC plate? Explain your answer clearly.What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to visualize a TLC plate?An example of planar chromatography is: Column chromatography HPLC GLC TLC
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- Which type of chromatography is most useful for determining the concentrations of minerals in bottled water? size exclusion ion-exchange gas thin layer adsorptionA spectrophotometric method for the quantitative determination of the concentration ofPb2+ in blood yields an Ssamp of 0.133 for a 1 mL sample of blood that has been diluted to 6 mL. A second sample is spiked with 1 µL of a 1467 ppb Pb2+ standard and diluted to 6 mL, yielding an Sspike of 0.491. Determine the concentration of Pb2+ in the original sample of blood.Although TLC is qualitative and tells you what is present in a given sample, it can also be used to determine where a compound might be lost: What samples could you test on a single TLC plate that might give you this information?