Consider the Michaelis-Menten enzymes below and answer the following questions.Kcat (s')9.5*105 1.4*10*2.5*102 1.0*1075.0*10 8.0*10²Enzyme Km (M)AВa. Which enzyme has the highest affinity substrate? How do you know?b. Which enzyme can convert the most substrate to product in a given period of time? Howdo you know?c. Which enzyme has the highest catalytic efficiency? How do you know?

Enzymes are protein molecules that increase the rate of reaction by decreasing the activation…

Answered: Consider the Michaelis-Menten enzymes…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTASE. The kinetics data are shown below: [Substrate, µM] Vo with DEDS (µM/min) Vo without DEDS (uM/min) 1.667 1.600 3.544 12.500 1.923 4.259 13.333 2.139 4.737 %3D 14.000 2.268 5.019 5.000 2.410 5.336 Using linear regression analysis, determine the values of Vmax and KM of the enzyme in the ABSENCE of inhibitor: (Express your answer in 3 decimal places, do NOT include the units) Vmax = KM=
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