(c) Based on the primary sequence surrounding the transmembrane portion, predict which Portion would likely be found on the cytoplasmic side of the membrane (if any), and which Portion (if any) would be found on the extra cellular side of the membrane according to The "inside positive" rule. Briefly justify your reasoning. (d) Predict the fragments that would be produced by digesting the 5-HT 24 Receptor protein With Trypsin. How might you use Trypsin to determine the orientation of the transmem- Brane protein?

(c) Based on the primary sequence surrounding the transmembrane portion, predict which Portion would likely be found on the cytoplasmic side of the membrane (if any), and which Portion (if any) would be found on the extra cellular side of the membrane according to The "inside positive" rule. Briefly justify your reasoning. (d) Predict the fragments that would be produced by digesting the 5-HT 24 Receptor protein With Trypsin. How might you use Trypsin to determine the orientation of the transmem- Brane protein?

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter32: The Reception And Transmission Of Extracellular Information

Section: Chapter Questions

Problem 3P

See similar textbooks

Similar questions

Psilocybin is rapidly de-phosphorylated in the body into psilocin, which is anagonist For the 5-HT 2A Serotonin receptor, which binds the neurotransmitter serotonin as its natural ligand, and acts as a ligand-gated ion channel in the central nervous system. HO. Serotonin (a) Part of the sequence of the 5-HT 24 Receptor is NH₂ ...RQKACKVIGIVFFLFVVMWLAFFITNICDES... Identify the trans-membrane region within this sequence. Do you predict this sequence will Be transmembrane a-Helix or ß sheet? Why? (b) What is the distance spanned by this transmembrane region?
Endocrine Ligand acting on intracellular receptor should be: O Hydrophilic O Hydrophobic O Amphipathic O Macromolecule What kinds of molecules may pass through
Arrange following the boxed information in sequence regarding the dissociation and reassociation of G proteins following the activation of a G protein coupled receptor. GTP-bound Ga, dissociates from B and y cuhunits Gas exchanges GTP for GDP Gas reassociates with By subunits and the rorentor reforms Binding of the ligand to a G-protein rounled recentor GTP-bound Gas binds and activates adenvlul rurlase GTP is hydrolyzed and GDP bound Gas suhunit dissoriates Conformational change Increased synthesis of CAMP from ATP
Ethanol is unusual in that it is freely soluble in both water and lipids. Thus, it has access to all regions of the highly vascularized brain. Although the molecular basis of ethanol action in the brain is not clear, ethanol evidently influences a number of neurotransmitter receptors and ion channels. Suggest a biochemical explanation for the diverse effects of ethanol.
GTPYS is a nonhydrolyzable analog of GTP. Suppose this compound were added to a cell-free system containing active components of an adrenergic signaling system. What consequences would you expect? What would be the effects on CAMP levels? || -0-P-o-P-0-P-o-CH, guanine он он GTPYS -
EGTA is a chelating agent with high affinity and specificity to Ca2+. By microinjecting a cell with an appropriate EGTA solution, an experimenter can prevent rising cytosolic Ca2+ from rising above 10-7 M. How would EGTA microinjection affect a cell’s response to the ?α-adrenergic and ?β-adenergic pathways?
There is an unusually high concentration of phosphatidylcholine on the lumenal side of the ER. What structural feature of phosphatidylcholine is responsible for this? Explainhow this structural feature produces this effect
Which of the following glucocorticoids is hydrocortisone and expected to have the LEAST binding affinity toward glucocorticoid receptor? НО A HO O D OH -OH НО OH OH В 1 O HO E OH OH OH ро CH3 С OH OH НО. с 55 -CH₂
All the B - adrenergic receptors are linked with Gs G-protein. Physiological processes mediated by the intracellular second messenger cyclic AMP include: YOU CAN CHOOSE MORE THAN ONE A.carbohydrate breakdown by the liver (glycogenolysis) B.increased contractility (e.g. increased cardiac contractility) C.smooth muscle relaxation (e.g. bronchodilation or vasodilation) D.Triglyceride breakdown (lipolysis)
Part A Which of the following statements about pain-relief substances is correct? View Available Hint(s) O Endorphins are synthetic pain-killers. O Met-enkephalin has pain-killing ability because of its structural similarity to morphine. O Many natural pain-killing polypeptides are ideal for medical use. O Receptors of opium derivatives are found in all body cells. Submit
Why is it that muscarinic ACh receptors can depolarize some cells and hyperpolarize others? There are different muscarinic ACh receptor subtypes - they are ionotropic receptors and activate different signaling pathways. O There are different muscarinic ACh receptor subtypes - they are ionotropic receptors and open different ion channels. There are different muscarinic ACh receptor subtypes - they are metabotropic receptors and activate different signaling pathways. There are different muscarinic ACh receptor subtypes - they are metabotropic receptors and open different ion channels. None of the above - muscarinic ACh receptors can only depolarize cells
The NMDA receptor I/V curve is nonlinear, why? What about the GluA2-containing AMPA receptor I/V curve? Draw both under normal physiological conditions. In addition, explain what happens for the NMDA receptor I/V curve when you lower the magnesium concentration.