B K Exercise 1B - Using the compound microscope Match each part of the compound microscope on the left with its function on the right: base and arm A. eyepiece, what you look in to see an image coarse focus knob B. adjusts position of slide left/right, front/back condenser lens C. used to bring the image into sharp focus diaphragm D. flat surface on which slide is placed fine focus knob E. secures slide in place before viewing high power objective lens F. focuses light from the lamp on the slide lamp G. used only with the low power objective low power objective lens H. used to handle the microscope properly mechanical stage knobs I. adjusts the amount of light passing through slide ocular lens J. used when you first examine a slide stage K. light source used to illuminate specimen stage clip L. used to produce a more magnified image

B K Exercise 1B - Using the compound microscope Match each part of the compound microscope on the left with its function on the right: base and arm A. eyepiece, what you look in to see an image coarse focus knob B. adjusts position of slide left/right, front/back condenser lens C. used to bring the image into sharp focus diaphragm D. flat surface on which slide is placed fine focus knob E. secures slide in place before viewing high power objective lens F. focuses light from the lamp on the slide lamp G. used only with the low power objective low power objective lens H. used to handle the microscope properly mechanical stage knobs I. adjusts the amount of light passing through slide ocular lens J. used when you first examine a slide stage K. light source used to illuminate specimen stage clip L. used to produce a more magnified image

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.10: The Microscope

Problem 18RQ

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PART C: CALCULATING THE DIAMETER OF THE FIELD OF VIEW (FOV)_ The field of view (FOV) is the circular area you can see when you look through the microscope. The diameter of the field of view is different depending on which objective lens you are using. For example, you are using the medium-power objective lens, then the area you can see is actually smaller than if you were using the low-power objective lens. Knowing The diameter of the field of view can help you estimate actual size of objects / cells seen through the microscope. When the revolving nose piece is turned to the low power objective lens, a dear plastic ruler can be placed on the microscope stage (see figure 1). Then, the coarse adjustment knob can be used to focus on the millimeter marks of the ruler making sure that one of the milimeter marks is at the left edge of the field of view (see figure 2). NOTE: Slage cip I-1000 Objects in the FOV are usually measured in micrometers (um). To convert, a FOV in mm, times it by 1000…
1. Match: Read about each microscope part. Match the description to the part on the diagram. Stage: Platform where a slide is placed. Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly magnifies a sample by 10x. Coarse focus knob: Large knob that moves the stage up and down to focus the sample. Fine focus knob: Small knob that moves the stage over a short distance to refine the focus. Objective lens: A second lens that further magnifies the sample. Microscopes usually have several objective lenses with different magnifications. Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.
Match the part of the microscope with its use. Dragged and dropped options on the right-hand side will be automatically saved. For keyboard navigation... SHOW MORE Stage Nosepiece Vertical Stage Knobs Course and Fine Adjustment Knobs Iris Diaphram III ||| Allows for changing objective lenses Move the stage side to side Hold the slide in place Control light passing through the stage Move the stage up and down
a. Why must you slowly turn the fine adjustment knob? b. Is natural light or is artificial light used when using the microscope?
What is the highest magnification of the following microscopes?Bright fieldDark fieldPhase-contrastFluorescenceConfocalScanning EMTransmission EMScanned-Probe
You have placed a microscope slide in a microscope and want to view it at with the 10X objective lens. List the steps that are necessary to set up and focus the microscope properly using the 10X objective lens.
You are in charge of buying new microscopes for your company, and you have been comparing different types of microscopes. One important property for microscopy is the resolution, which measures the ability to distinguish two small objects that are close together. Rank the following list of microscopes from lowest to highest resolution. Microscope List (5 items) (Drag and drop into the appropriate area) Transmission electron microscope (uses high energy electron beam) X-ray microscope (uses x-ray absorption to image sample, range 0.01-10 nm) Confocal fluorescence microscope (uses a blue laser as a light source, 488 nm) Level of Resolution 2 Lowest
1. Identify features of Dark-field Microscopy (1-2 paragraph) 2. Give three examples of the uses of microscopes outside the field of biology and medicine. (3 examples with 5sentences explanation each)
SBI 3C1 VIRTUAL LAB: THE MICROSCOPE INSTRUCTIONS: Go to the following link: https://virtuallabs.nmsu.edu/micro.php. Click the continue tab and follow the instructions on how to properly use a microscope. When you are complete, answer the questions below. PART A: MAGNIFICATION OF THE MICROSCOPE - How much biggerl enlarged is the specimen? TOTAL MAGNIFICATIION (eyepiece (ocular) magnification) X (objective lens magnification) Calculate the total magnification for each lens below for a simple COMPOUND LIGHT MICROSCOPE ОBJECTIVE LENS POWER OCULAR MAGNIFICATION OBJECTIVE LENS MAGNIFICATION TOTAL MAGNIFICATION MAG (X) = Ocular X Objective LOW LP MAG = MEDIUM MP MAG= HIGH HP MAG- Complete the following chart by calculating the missing lens or total magnification [2] TOTAL MAGNIFICATION OBJECTIVE LENS MAGNIR AR (EYEPIECE) MAGNIFICATION 5X 80X 10X 40X 10X 100X 500X 50X PART B: HOW TO USE THE COMPOUND MICROSCOPE TO VIEW SLIDES Access the Virtual Microscope at…
1. What does moving the coarse focus and fine focus do? Why is it important to remember the difference between coarse and fine focus? 2. What is the resolution of a microscope? How is resolution affected byadjusting the light, coarse and fine focus? please answer on ur own words, do not copy from google or i will downvote
3. Please number these steps, in the order they should be completed when using a microscope. Remove specimen, lower stage, wrap cord, and return covered microscope to numbered shelf. Move to other objective lenses to increase magnification. Rotate lowest-magnification objective lens into position (probably 4x or 10x). Clean all lenses with lens paper. Place specimen on stage. Adjust chair to a comfortable height. Use coarse focus knob to adjust stage until an image is visible. Be careful to use only the fine focus knob with the longest objective lenses.
USING & FOCUSING THE MICROSCOPE: 1. List the following items into their correct order for proper set-up & use of the microscope: set scanning lens (4x), set low power lens (10x), turn on power, place slide on stage, set light to medium, look through eyepiece (= oculars), fine adjustment focus, coarse adjustment focus, center specimen 1. 2. 3. 4. 5. 6. 7. 8. 9.