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Topic: Preparation of Microbial Suspension
A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included.
Effect:
Rationale:
Step by step
Solved in 2 steps
- Topic: Spread-Plate Method Agar was not completely set prior to inoculating with the organism. Effect:Rationale:Topic: Preparation of Microbial Suspension 5. Over-dilution with sterile water. Effect: Rationale:Topic: Preparation of Microbial Suspension 4. Inaccurate volume measurements of aliquots during dilution. Effect: Rationale:
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient AgarPlastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:
- NOTE: Kindly provide references. 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and gamma-hemolytic reactions on blood agar media (BAM). Cite two (2) examples of bacteria causing the above hemolytic reactions.Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus) CAMP Test Coagulase Tube Test PYR Test Novobiocin Sensitivity TestLid of petri dish was left on bench top for a few minutes after pouring of sterile medium. Effect: Rationale: