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- 1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.A Br selective electrode is applied for measuring bromide ions in saline solution. Cl' ions were found to interfere, with a kPot = 0.0050. %3D After calibration, the ISE equation was determined to be y = -70 mV - 59.2mV*log(x). If we measure a potential of 93 mV, and the [CI]=0.100 M, what was the [Br'] in units of M? O 0.00821 O 0.100 O 0.0050 O 0.123 O Correct answer not found in other optionsSolution A is 20 degrees celsius, Solution B is 80 degrees celsius (both are the same kind of solution). The goal is to make a 50 degree celsius solution; How much of solution A do you need (starting with 100g of solution B)? (cp=4.184 J/g C)
- A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).Consider a suspension of particles (isoelectric point is at pH 6) in water at pH 2 and a NaCl concentration of 0.001 M. Describe how the strength of repulsion varies with the following changes, assuming all other conditions remain constant. Give a description (more than just increase or decrease) in terms of the effect on the double layer thickness and the zeta potential. (a) Change from 0.001 M NaCl to 0.1 M NaCl, (b) Change from pH = 2 to pH = 5.Guanosine (C10H13N5O5) in solution has a maximum absorbance at a wavelength of 275 nm. The molar extinction co-efficient at this wavelength is 84M−1cm−1and the path length is 24.7 cm. Through the use of a spectrophotometer, it is found that the that A275= 1.48. What is the concentration of the guanosine solution in grams/litre? Molecular weights (g/mol): C-12, H-1, N-14, O-16 Select one: A. 0.201869 g/L B. 0.435188 g/L C. 0.059919 g/L D. 0.294046 g/L E. 0.000713 g/L
- At what concentration of S(expressed as a multiple of KM) will νo=0.95Vmax?Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured with the spectrophotometer to have an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? 0.9 0.8 0.7 0.6 805 4 0.4 0.3 0.2 Absorbance at 540 mWhat is the frequency of green light that has a wavelength of 531 nm? (c = 3.00 x 10⁸ m/s)
- Can you please calculate the following?The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.Calculate the average dipole–induced-dipole interaction energy, in joules per mole (J mol−1), between a water molecule and a benzene molecule separated by 1.0 nm.