3. Hershey-Chase (1952) The Definitive Proof Hershey and Chase utilized phages in their research. A bacteriophage is a virus that infects a bacterium, it consists of a protein coat and DNA, it also reproduces quickly and can easily be harvested. Hershey and Chase used radioactive P and S to track the differences in the protein and DNA, since only DNA contains P and only proteins contain S. They infected bacteria with the phage that had the radioactive S, and they infected another group of bacteria with a radioactive P. The results showed that the bacteria contained radioactive P entered the bacterium. Watch this to help you understand: https://www.youtube.com/watch?v=hubXqPrsIR4 EXPERIMENT Radioscthe Kaioactivity Page

3. Hershey-Chase (1952) The Definitive Proof Hershey and Chase utilized phages in their research. A bacteriophage is a virus that infects a bacterium, it consists of a protein coat and DNA, it also reproduces quickly and can easily be harvested. Hershey and Chase used radioactive P and S to track the differences in the protein and DNA, since only DNA contains P and only proteins contain S. They infected bacteria with the phage that had the radioactive S, and they infected another group of bacteria with a radioactive P. The results showed that the bacteria contained radioactive P entered the bacterium. Watch this to help you understand: https://www.youtube.com/watch?v=hubXqPrsIR4 EXPERIMENT Radioscthe Kaioactivity Page

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter22: Prokaryotes: Bacteria And Archaea

Section: Chapter Questions

Problem 25RQ: MRSA has emerged as a serious infectious disease, with the first case of methicillin-resistant S....

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HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. How did the researchers know that the radioisotopes in the fluid came from outside of the bacterial cells and not from bacteria that had been broken apart by whirling in the blender?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. The extracellular concentration of which isotope increased the most with blending?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Before blending what percentage of each isotope. 35S and 32P, was extracellular (outside the bacteria)?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Do these results imply that viruses inject DNA or protein into bacteria? Why or why not?
Row C D. B B. one: A C O phage with radiolabelled protein coat phage with radiolabelled DNA 100 10. phage infects The experiment shown above was designed by bacterium phage infects bacterium EXPERIMENT 1 phage shell is removed EXPERIMENT 2 요 Hershey & Hershey & Chase Meselson & Stahl Meselson & Stahl 8 phage shell is removed 28 no radioactivity in cells ii 48 radioactivity in cells LL and proved that ii DNA replication is semiconservative DNA is the hereditary material DNA replication is semiconservative DNA is the hereditary material (select the row that correctly completes the statement)
Bacterial transformation is a major concern in many medical settings. Why might health care providers be concerned? Pathogenic bacteria could introduce disease-causing genes in non-pathogenic bacteria Antibiotic resistance genes could be introduced to new bacteria to create “superbugs. ” Bacteriophages could spread DNA encoding toxins to new bacteria All of the above.
MRSA has emerged as a serious infectious disease, with the first case of methicillin-resistant S. aureus being detected in 1961. Why are medical professionals so concerned when antibiotics exist that can kill MRSA? MRSA can transfer methicillin-resistance to other bacteria Patients are not treated with correct antibiotics rapidly enough to prevent serious illness MRSA could acquire additional antibiotic resistance genes from other bacteria to become a “super bug." All of the above.
What is required for the transformation of a bacterial cell (as opposed to conjugation and transduction)? The creation of a strand of DNA from an RNA molecule The infection of cells by a phage DNA molecule The synthesis of a cytoplasmic bridge between two bacterial cells. O Uptake of DNA by the cell, usually by heat shock treatment in the lab. fg f1o 7 7. 8
Conjugation results in the transfer of genetic material between two bacterial cells, but only occurs if one bacterial cell is O F+ Conjugated Transformed O F-
Refer to the following illustration to answer the question_ The illustration shows: O a lysogenic phage O a lytic phage new DNA O replicative transposition sequence non-replicative transposition O site-specific recombination
The process of transferring DNA from one bacterium to another through a bacteriophage is called O conjugation O the lysogenic cycle O transformation O transduction O the lytic cycle 0|00
9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation method Description Ligated product incubated with competent E. coli, heat-shock treatment. Cells incubated with the plasmid DNA are subjected to a high-voltage pulse. Transiently renders the bacterial membrane permeable to large molecules. The process of injection of foreign DNA into a cell using micropipette/micromanipulator.