#2 Mol Bio Mutation QUESTIONS - Saved to this PC - P Search A MSTMCHUNU MT sert Design Layout References Mailings Review View Help Chemistry 8 Share P Comments E It looks like your stored credentials are out of date. Please sign in as 20******@ke****** za so we can verify your subscription. Sign In Task B [5 points]: Sanger sequencing Having successfully amplified the NRAS gene from wild-type and cancerous tissue through PCR, you now sent your PCR product for sequencing. However, the only available technology to you is classical Sanger sequencing or the dideoxy chain termination method, explained in Figure 2. Shown below are the sequencing gels obtained for the sections of the wild-type and mutant NRAS genes where the mutation can be found. WILD-TYPE NRAS MUTANT NRAS ddA ddT ddc ddG ddA ddT ddC ddG 1. Determine the 5'-to-3' sequence of the wild-type TEMPLATE strand. Answer: 5' 3' 2. Using the codon table given below, determine the corresponding wild-type and mutant amino acid sequences in order to determine the mutation that occurred. Assume that the sequencing template is the CODING strand for the NRAS gene. (Use the 3-letter abbreviations for the amino acids.) Second E English (United States) O Focus 100% ENG 17:34 re to search A 22°C A a dx
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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Task B [5 points]: Sanger sequencing
Having successfully amplified the NRAS gene from wild-type and cancerous tissue through PCR,
you now sent your PCR product for sequencing. However, the only available technology to you
is classical Sanger sequencing or the dideoxy chain termination method, explained in Figure 2.
Shown below are the sequencing gels obtained for the sections of the wild-type and mutant NRAS
genes where the mutation can be found.
WILD-TYPE NRAS
MUTANT NRAS
ddA
ddT
ddC
ddG
ddA
ddT
ddC ddG
1. Determine the 5'-to-3' sequence of the wild-type TEMPLATE strand.
Answer: 5'
3'
2. Using the codon table given below, determine the corresponding wild-type and mutant amino
acid sequences in order to determine the mutation that occurred. Assume that the sequencing
template is the CODING strand for the NRAS gene. (Use the 3-letter abbreviations for the amino
acids.)
Second letter
UGUT
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E English (United States)
D Focus
100%
445 words
ENG
17:34
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W
22°C
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