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- do methodologies preparation of the following 6 solutions (Broth LB, LB Agar, Potato-Dextrose Agar, LB-Dextrose Broth, Saline solution 0.85%, Glycerol 80%). Include photos or schematics.&. How many milldiners of a 2wv Lidocaine HCI stock selution in water should be used in preparing 30 ml of a 0.5% wiv Lidocaine HCI solution? . From the following formula for antificial tears, caleulate the quantity of each ingrodient roquired to prepare half donen of the 0ml containers. Poyviny alcohol 149 Povidone Chiorobutanol Serile sodum chioride soution a.%. s to 100 m How many milldiners of water should be added to 60 ml. of a 2% wiv agqueous solution to prepare a 1% wiv solution?Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungi
- Which can infect hur Table 1: Absorbance and appearance of broth tubes at various temperatures. E. colin B. stearothermophilus Temperature°C Absorbance Visual Absorbance Visual 4 0.04 Clear 0.06 Clear tre Light turbid Very turbid Almost clear 25 0.66 0.09 Clear Light turbid Very turbid 37 1.48 0.55 which 55 0.25 1.29 oledzethe mes he alhe ive to edeut et brahell bras atnstooinieib o ho leviviue zwolls ti saunMatch inhibitors, selective ingredients, pH indicators and other components to correct medium phenol red pH indicator [ Choose ] [Choose] mannitol Мас Sabouraud Agar 7.5 g NaCI/100 ml inhibitor Blood agar MSA low pH, high dextrose concentration [ Choose ] inhibitors neutral red pH indicator [ Choose ] crystal violet and bile salt inhibitors [ Choose] no inhibitors [Choose ] lactose as only sugar in medium [ Choose ]AY AY * 00 HI T 00 2 3. ment10: Connect Regis.. M https://Ims.mh.. nemical Content of Media i Saved Help Save & Exit Chec Chemically defined medium Complex medium 2 Enriched medium Fastidious Heterotroph 9 Autotroph Match each of the options above to the items below. Uses inorganic carbon as a carbon source for building its own organic molecules. A type of organism that has complex nutrient requirements and requires a nutritionally dense environment. A media containing extracts from other other organisms. A medium with specifically added nutrients to enhance the growth of fastidious organisms. An organism that uses organic carbon as its carbon source. A medium of which the exact chemical composition is known. MacBook Air DD F7 000 000 F2 F4 F5 F8 %23 2$ 4. 9- } 1 Y | 7.
- Based on the following image, which concentration of antibiotic would be considered the minimum bactericidal concentration (MBC)? a. 8 ug/mlb. 16 ug/mlc. 25 ug/mlTime point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.Please help me solve this experiencee İSTINYE İSÜ ÜNİVERSİTESİ Peptone peptme extrcr İSTİNYE İÜ ÜNİVERSİTESİ LB Boh 5.00d 5.000 STA 2.5L07 LB Barth The apecinet: your LB agar. LB Boxth 2.501 extlact yenst 5.0067 NCl is e Solid media L8 g LB agar 5.0130 Nad Sooml dispillek waher Sg peprone 25g yeast exeracb. Nacl We add 400 ml wkter and mix the mixtule, then. the ph value, before Yest of the amOun. thet we will add Sooml messuing 59 A the ee. no need for heat when we aie miy tuting the Solution magnatic 3 H S.ooL pepane ve Used the Some spAn to put the peprone onAlsmiaure sheea Slale 5.000
- a physician prescribed an antibiotic to be mixed in white petrolatum to produce a 25% antibiotic preparation. The pyscian later changed the protocol to be only 12.5%. How much white petrolatum must be mixed with each 6-oz ointment container of 25% prepartation to make the new 12.5% preparation? Calculate answer in grams. Answer should be 180gdiameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?