Preparing Disinfectant To Clean E. Coli

Three ways to assess water are positive/negative, membrane filtration and IDEXX Colilert and Enterolert assays. For membrane filtration, petri dishes and an incubator are used. For IDEXX coliert powdered medium is used for total coliform and E. Coli, while Enterolert media is used for enterococci. Also an incubator, colilert trays are used. IDEXX is more expensive than membrane filtration.

It is also important to keep learning about the new disinfectants that come out; there are constant upgrades and improvements being made with these products. There are so many different types of disinfectants so it’s important for you to choose the one that’s best suited for you. When looking for a disinfectant it’s also important to pick one that has an efficacy claim on the label. Efficacy means that it is able to produce and effect, it is also the effectiveness of the products ability to kill organisms when used correctly. Disinfectants also require upkeep; you have to be sure to change after the period of time recommended by the manufacturer. It is important to always wear gloves and safety glasses when handling disinfectant solutions. You should always keep an MSDS for the disinfectant, and always use tongs when removing items from disinfectant. You never want to let a disinfectant come in contact with your skin, if you ever do you should immediately wash with soap and warm water, rinse, and dry thourghly. Always dilute products as specified of the instructions on the label. The most common disinfectant in spas is Quaternary ammonium compounds, or “Quats”. Quats usually disinfects in ten minutes, some contain antirust ingredients so if you leave tools in the solution for too long it can cause dulling or damage. After they are in the solution for the specified time you should rinse, dry, then store in a clean, covered

“Ajax Spray ‘n’ Wipe” specifically states that it kills E. coli. This is because of the lactic acid in the spray, which is used as a natural antibacterial agent.” ("Colgate-Palmolive | Product Ingredients"). Lactic acid kills unwanted bacteria especially in food which is where E. coli is often found and transmitted, of which meat is the most common cause. Vinegar was chosen as a substance because “it can be used as a bleach alternative” (“Disinfectants: A Guide to Killing Germs the Right Way”) and bleach kills E. coli. It is also used as an everyday cleaning product. The last substance chosen was salty water because it is thought that salt kills bacteria. Much of the world’s population has access to salt and if the experiment proves that salt kills E. coli it would be a helpful factor for further treatment of the

room temperature. Vials 4, 5, and 6 should be in the bath containing water that is 10oC. After the

4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.

E.coli outbreaks have steadily grown over the last few decades. An expansion in big farming has led to E. coli not only being found in meat, but vegetation as well, due to waste runoff. This has increased our need for adequate antibiotics that can fight bacteria, like E. coli. The best way to pinpoint which antibiotics work is by measuring their ability to create antimicrobial agents or zones of inhibition. When a paper disc that has been saturated in an antibiotic is inserted in a solution of E.coli and medium, the zone of inhibition will be noted as the clear ring that forms around the disk. The antibiotics efficacy is then determined by measuring each disk zone of inhibition, and comparing these measurements to the zone measurements of an untreated specimen. If an antibiotic is to be deemed sufficient for treating E. coli it should show a zone of inhibition that is at least double the size of the untreated specimen.

8. With the Petri dishes on a flat surface, open sterile applicators and touch the applicators to the Agar surface to obtain a sample

Purpose: To explore the effectiveness of different levels of concentration of antiseptic and antibacterial products’ prevention of bacterial growth.

Have you ever wondered why people are always talking about E. coli, what it is, and how you can get it? What are the conditions this bacteria lives in? These interesting questions led the investigator to find out which material inhibits E. coli replication in an aqueous environment the most.

Lightly move the loop across the top of the agar to collect the bacteria. Flame the neck again and replace the cap. 5. Use the loop to mix the bacteria with the

We used forceps to dispense the antibacterial disks to the specific quadrants on each plate (refer to the lab handout to see what disks were used for each individual quadrant). We dispensed hand sanitizer on the test solution quadrant for plate 3.

Transfer the vial contents into the warmed media and centrifuge at 1000 rpm x 5 min.

Chlorine is one of the most widely used disinfectant. It is very effective for the deactivation of pathogenic microorganisms. Chlorine can be easily applied, measured and controlled. It is also relatively inexpensive.

The flaw with the design of this experiment subsists that the liquid tested (Dettol Surface Cleaner) is in fact disinfectant and not a steriliser, meaning that although it kills most germs (therefore being a disinfectant), it does not kill all germs (therefore not being a steriliser) as it has deficiencies in its sporicidal properties because it’s killing ‘techniques’ are cell dehydration, membrane disruption, and protein coagulation, these along with Hydrophilic Viruses (viruses which are mixed with another liquid, eg; water) also can also be resistant. Although E. Coli is hydrophobic, if any air transmitted viruses were to settle on the agar, the results would be extremely altered.

Re-flame the neck of the bottle and place the top on. Place the disc on section 1A of the agar plate. 3. Repeat step 2 for the rest of the petri dishes (1B, 1C, and so on) 4. Place the petri dish 1 in the incubator at a temperature of 50 degrees Celsius, and leave for 24 hours exactly.